Mammalian Genome

, Volume 20, Issue 3, pp 140–151

Genetic analyses reveal a requirement for Dicer1 in the mouse urogenital tract

  • Laura M. Pastorelli
  • Sara Wells
  • Martin Fray
  • Adrian Smith
  • Tertius Hough
  • Brian D. Harfe
  • Michael T. McManus
  • Lee Smith
  • Adrian S. Woolf
  • Michael Cheeseman
  • Andy Greenfield
Article

DOI: 10.1007/s00335-008-9169-y

Cite this article as:
Pastorelli, L.M., Wells, S., Fray, M. et al. Mamm Genome (2009) 20: 140. doi:10.1007/s00335-008-9169-y

Abstract

Despite the increasing interest in other classes of small RNAs, microRNAs (miRNAs) remain the most widely investigated and have been shown to play a role in a number of different processes in mammals. Many studies investigating miRNA function focus on the processing enzyme Dicer1, which is an RNAseIII protein essential for the biogenesis of active miRNAs through its cleavage of precursor RNA molecules. General deletion of Dicer1 in the mouse confirms that miRNAs are essential for development because embryos lacking Dicer1 fail to reach the end of gastrulation. Here we investigate the role of Dicer1 in urogenital tract development. We utilised a conditional allele of the Dicer1 gene and two Cre-expressing lines, driven by HoxB7 and Amhr2, to investigate the effect of Dicer1 deletion on both male and female reproductive tract development. Data presented here highlight an essential role for Dicer1 in the correct morphogenesis and function of the female reproductive tract and confirm recent findings that suggest Dicer1 is required for female fertility. In addition, HoxB7:Cre-mediated deletion in ureteric bud derivatives leads to a spectrum of anomalies in both males and females, including hydronephrotic kidneys and kidney parenchymal cysts. Male reproductive tract development, however, remains largely unaffected in the absence of Dicer1. Thus, Dicer1 is required for development of the female reproductive tract and also normal kidney morphogenesis.

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Laura M. Pastorelli
    • 1
  • Sara Wells
    • 2
  • Martin Fray
    • 1
  • Adrian Smith
    • 2
  • Tertius Hough
    • 2
  • Brian D. Harfe
    • 3
  • Michael T. McManus
    • 4
  • Lee Smith
    • 5
  • Adrian S. Woolf
    • 6
  • Michael Cheeseman
    • 1
    • 2
  • Andy Greenfield
    • 1
  1. 1.Mammalian Genetics UnitMRC HarwellOxfordshireUK
  2. 2.The Mary Lyon CentreMRC HarwellOxfordshireUK
  3. 3.Department of Molecular Genetics and MicrobiologyUniversity of Florida College of MedicineGainesvilleUSA
  4. 4.Department of Microbiology and Immunology Diabetes CenterUniversity of CaliforniaSan FransiscoUSA
  5. 5.MRC Human Reproductive Sciences UnitThe Queen’s Medical Research InstituteEdinburghUK
  6. 6.Nephro-Urology UnitUCL Institute of Child HealthLondonUK

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