Mammalian Genome

, Volume 19, Issue 7–8, pp 581–586

piRNA-like RNAs in the marsupial Monodelphis domestica identify transcription clusters and likely marsupial transposon targets

Article

Abstract

PIWI-interacting RNAs (piRNAs) are a recently discovered class of small noncoding RNAs that have been detected in human, mouse, rat, zebrafish, and Drosophila genomes. We have utilized a size-directed small-RNA cloning procedure to clone and map more than 300 candidate piRNA-like small RNAs in the genome of the marsupial species Monodelphis domestica. Our results are consistent with those from other species in that the piRNA-like candidate sequences range in size from 28 to 31 nucleotides, show a pronounced preference for uridine at the 5′ end, are transcribed from a few large clusters, appear to target transposons, and display virtually no sequence conservation.

Supplementary material

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335_2008_9109_MOESM2_ESM.doc (22 kb)
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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Eric J. Devor
    • 1
  • Lingyan Huang
    • 1
  • Paul B. Samollow
    • 2
  1. 1.Molecular Genetics and BiophysicsIntegrated DNA TechnologiesCoralvilleUSA
  2. 2.Department of Veterinary Integrative BiosciencesTexas A&M UniversityTXUSA

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