Discovery of a new HBB haplotype w2 in a wild-derived house mouse, Mus musculus
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Genetic characterization of a wild-derived house mouse, Mus musculus, originally collected near Lake Balkhash in the Republic of Kazakhstan, was performed by examining protein polymorphisms and nucleotide sequences for the hemoglobin beta chain (HBB) subunits. Protein electrophoresis, which was performed on a cellulose-acetate plate, showed an independent mobility pattern representing a new, previously undiscovered haplotype. Neighbor-joining analyses of the HBB adult genes, i.e., HBB-T1 and HBB-T2, and the intergenic spacer region showed that the Lake Balkhash mouse possessed genomic components that were mixed from different haplotypes. Compared to the previously determined HBB haplotypes, d, p, and w1, the HBB-T1 gene and ca. 11 kb of the spacer region were most similar to the w1 haplotype; however, the remainder of the spacer region and the HBB-T2 gene were most similar to the d haplotype but may represent a still uncharacterized and divergent haplotype. The recombination event is predicted to have occurred 2.5 kb upstream of the HBB-T2 gene and may have occurred through intersubspecific hybridization between mice of the musculus subspecies group (with the w1 haplotype) and the castaneus subspecies group (with the d-like haplotype). Alternatively, an unknown subspecies group that is equidistantly divergent from each of these subspecies groups may have been involved. Our findings suggest reticulate evolution among the subspecies groups during the evolution of M. musculus.
KeywordsRecombination Event Wild Mouse Iodoacetic Acid Intergenic Space Region Inbred Laboratory Strain
The authors thank Kumi Futawaka, Mihoko Hatase, Kyoko Hirai, Hatuyuki Hirata, Kazuyuki Mekada, Yuuki Shichi, Miho Shimura, Yoshiharu Tsuru, Junpei Ueta, and Daisuke Watanabe for their technical support. The animals and experiments were partially supported by the Presidential Grant of the University of Miyazaki for encouragement of young scientists (for AS). This work was supported by RIKEN BRC (Director, Yuichi Obata) and in part by a grant-in-aid from the Ministry of Education, Science, Sports and Culture of Japan (for KM, HY, and YY).
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