Mammalian Genome

, Volume 18, Issue 5, pp 338–346 | Cite as

Generation of rat mutants using a coat color-tagged Sleeping Beauty transposon system

  • Baisong Lu
  • Aron M. Geurts
  • Christophe Poirier
  • Deborah C. Petit
  • Wilbur Harrison
  • Paul A. Overbeek
  • Colin E. Bishop


A significant barrier to exploiting the full potential of the rat as a biomedical model is the lack of tools to easily modify its germline. Here we show that a tyrosinase-tagged Sleeping Beauty transposon can be used as a simple, efficient method to generate rat mutants in vivo. By making two lines of transgenic rats, one carrying the transposon and another expressing the transposase in germ cells, we are able to obtain bigenic males in which transposition occurs in the germ cells. We show that transposition leads to the appearance of new coat colors in the offspring. Using such bigenic males, we obtained an average of 1.2 transpositions per gamete and identified 19 intragenic integration events among 96 transposition sites that were sequenced. In addition, gene trapping was confirmed and rats with evidence for transposon-induced dominant ocular anomalies were identified. These data suggest that the modified Sleeping Beauty transposon represents a powerful new tool for producing molecularly defined mutagenesis in the rat.


Coat Color Splice Acceptor Sleep Beauty Transposition Event Gene Trapping 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors thank Carolyn Castile and Helen Martinez for excellent technical support. This work was funded by NIH grant R21RR021947 to CEB.


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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Baisong Lu
    • 1
    • 2
  • Aron M. Geurts
    • 3
  • Christophe Poirier
    • 1
    • 5
  • Deborah C. Petit
    • 1
    • 6
  • Wilbur Harrison
    • 4
  • Paul A. Overbeek
    • 4
  • Colin E. Bishop
    • 1
    • 2
  1. 1.Department of Obstetrics and GynecologyBaylor College of MedicineHoustonUSA
  2. 2.Institute for Regenerative MedicineWake Forest University Health SciencesWinston-SalemUSA
  3. 3.Human and Molecular Genetics CenterMedical College of WisconsinMilwaukeeUSA
  4. 4.Department of Molecular and Cellular BiologyBaylor College of MedicineHoustonUSA
  5. 5.Department of PediatricsUniversity of ChicagoChicagoUSA
  6. 6.Department of Veterinary Medicine and SurgeryUniversity of Texas, M.D. Anderson Cancer CenterHoustonUSA

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