Mammalian Genome

, Volume 17, Issue 12, pp 1193–1204 | Cite as

Further studies on using multiple-cross mapping (MCM) to map quantitative trait loci

  • Barry Malmanger
  • Maureen Lawler
  • Shannon Coulombe
  • Rochelle Murray
  • Staci Cooper
  • Yekaterina Polyakov
  • John Belknap
  • Robert HitzemannEmail author


We have completed whole-genome scans for quantitative trait loci (QTLs) associated with acute ethanol-induced activation in the six F2 intercrosses that can be formed from the C57BL/6J (B6), DBA/2J (D2) , BALB/cJ (C), and LP/J (LP) inbred strains. The goal was to test the hypothesis that given the relatively simple structure of the laboratory mouse genome, the same QTLs will be detected in multiple crosses which in turn will provide support for the strategy of multiple-cross mapping (MCM). QTLs with LOD scores greater than 4 were detected on Chrs 1, 2, 3, 8, 9, 13, 14, and 16. Only for the QTL on distal Chr 1 was there convincing evidence that the same or at least a very similar QTL was detected in multiple crosses. We also mapped the Chr 2 QTL directly in heterogeneous stock (HS) animals derived from the four inbred strains. At G19 the QTL was mapped to an approximately 3-Mbp interval and this interval was associated with a haplotype block with a largely biallelic structure: B6-L:C-D2. We conclude that mapping in HS animals not only provides significantly greater QTL resolution, at least in some cases it provides significantly more information about the QTL haplotype structure.


Haplotype Structure Multiple Cross Laboratory Mouse Strain Ethanol Response Heterogeneous Stock Population 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This study was supported in part by grants from the National Institutes of Health AA11034, AA 13484, MH 51372, and the Veterans Affairs Research Service.


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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Barry Malmanger
    • 1
    • 2
  • Maureen Lawler
    • 1
    • 2
  • Shannon Coulombe
    • 1
    • 2
  • Rochelle Murray
    • 1
    • 2
  • Staci Cooper
    • 1
    • 2
  • Yekaterina Polyakov
    • 1
    • 2
  • John Belknap
    • 1
    • 2
  • Robert Hitzemann
    • 1
    • 2
    • 3
    Email author
  1. 1.Department of Behavioral NeuroscienceOregon Health & Science UniversityPortlandUSA
  2. 2.Research Service, Veterans Affairs Medical CenterPortlandUSA
  3. 3.Department of Behavioral Neuroscience, L-470Oregon Health & Science UniversityPortlandUSA

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