The human FK506-binding proteins: characterization of human FKBP19
- 503 Downloads
Analysis of the human repertoire of the FK506-binding protein (FKBP) family of peptidyl-prolyl cis/trans isomerases has identified an expansion of genes that code for human FKBPs in the secretory pathway. There are distinct differences in tissue distribution and expression levels of each variant. In this article we describe the characterization of human FKBP19 (Entrez Gene ID: FKBP11), an FK506-binding protein predominantly expressed in vertebrate secretory tissues. The FKBP19 sequence comprises a cleavable N-terminal signal sequence followed by a putative peptidyl-prolyl cis/trans isomerase domain with homology to FKBP12. This domain binds FK506 weakly in vitro. FKBP19 mRNA is abundant in human pancreas and other secretory tissues and high levels of FKBP19 protein are detected in the acinar cells of mouse pancreas.
KeywordsFK506 Image Clone Secretory Tissue PPIase Domain FKBP Domain
This project was funded by a BBSRC CASE award sponsored by Pfizer Global Research and Development, Sandwich, UK. The authors thank Dr. Valerie Lucas and Richard Bazin at Pfizer UK for their assistance.
- Arakawa H, Nagase H, Hayashi N, Fujiwara T, Ogawa M, et al. (1994) Molecular cloning and expression of a novel human gene that is highly homologous to human FK506-binding protein 12kDa (hFKBP-12) and characterization of two alternatively spliced transcripts. Biochem Biophys Res Commun 200, 836–843CrossRefPubMedGoogle Scholar
- Davis EC (2000) In Immunophilins in the Brain, B. Gold, G. Fischer, and T. Herdegen, eds. (Barcelona: Prous Science), Fk506-Binding proteins in the Secretory pathway. pp 59–66Google Scholar
- Galat A, Riviere S (1998) Peptidyl-prolyl cis/trans isomerases. (Oxford: Oxford University Press)Google Scholar
- Goessling E, Kel-Margoulis OV, Kell AE, Wingender E (2001). MATCHTm- A tool for searching transcription factor binding sites in DNA sequences. Application for the analysis of human chromosomes. German Conference on Bioinformatics, Braunschweig, Germany (Oct 7–10, 2001)Google Scholar
- Ikeda Y, Schultz LW, Clardy J, Schreiber SL (1994) Structural basis for peptidomimicry by a natural product Journal of the American Chemical Society, 116, 4143–4144Google Scholar
- Kay JE (2000) FKBP ligands: Novel Strategies for the Treatment of Neurodegenerative Disorders. In Immunophilins in the Brain, B. Gold, G. Fischer, T. Herdegen, eds., (Barcelona: Prous Science), pp 263– 268Google Scholar