Mammalian Genome

, Volume 16, Issue 12, pp 955–962

Linkage analysis and gene expression profile of pancreatic acinar atrophy in the German Shepherd Dog

  • Leigh Anne Clark
  • Jacquelyn M. Wahl
  • Jörg M. Steiner
  • Wenli Zhou
  • Wan Ji
  • Thomas R. Famula
  • David A. Williams
  • Keith E. Murphy
Article

DOI: 10.1007/s00335-005-0076-1

Cite this article as:
Clark, L.A., Wahl, J.M., Steiner, J.M. et al. Mamm Genome (2005) 16: 955. doi:10.1007/s00335-005-0076-1

Abstract

Pancreatic acinar atrophy (PAA) is a degenerative disease of the exocrine pancreas and is the most common cause of exocrine pancreatic insufficiency in the German Shepherd Dog. Analyses of inheritance have shown that a single gene segregating in an autosomal recessive fashion is causative for PAA. To date the gene and causative mutation have not been determined. To identify a region of interest and/or candidate genes, we conducted linkage and gene expression studies. Analysis of 384 microsatellite markers resulted in a maximum two-point LOD score of 2.5 for FH2107 on CFA03. We used an oligonucleotide array to generate gene expression profiles for normal and affected pancreata. It revealed 244 genes with greater than two-fold difference in expression levels. Five genes of interest were further assessed by TaqMan quantitative real-time RT-PCR that confirmed trends observed using the microarray. One gene, gp25L, located on CFA03, was found to be downregulated by more than 500-fold in affected pancreata and was further investigated as a candidate gene. Sequence data did not reveal a mutation in the coding sequence that segregates with PAA.

Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Leigh Anne Clark
    • 1
  • Jacquelyn M. Wahl
    • 1
  • Jörg M. Steiner
    • 2
  • Wenli Zhou
    • 3
  • Wan Ji
    • 3
  • Thomas R. Famula
    • 4
  • David A. Williams
    • 2
  • Keith E. Murphy
    • 1
  1. 1.Department of Pathobiology, College of Veterinary Medicine and Biomedical SciencesTexas A&M UniversityUSA
  2. 2.Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical SciencesTexas A&M UniversityUSA
  3. 3.ViaGen, Inc.AustinUSA
  4. 4.Department of Animal ScienceUniversity of California DavisDavisUSA

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