Transcriptome analysis of human gastric cancer
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To elucidate the genetic events associated with gastric cancer, 124,704 cDNA clones were collected from 37 human gastric cDNA libraries, including 20 full-length enriched cDNA libraries of gastric cancer cell lines and tissues from Korean patients. An analysis of the collected ESTs revealed that 97,930 high-quality ESTs coalesced into 13,001 clusters, of which 11,135 clusters (85.6%) were annotated to known ESTs. The analysis of the full-length cDNAs also revealed that 4862 clusters (51.7%) contained at least one putative full-length cDNA clone with an initiation codon, with the average length of the 5′ UTR of 140 bp. A large number appear to have a diverse transcription start site (TSS). An examination of the TSS of some genes, such as TEGT and GAPD, using 5′ RACE revealed that the predicted TSSs are actually found in human gastric cancer cells and that several TSSs differ depending on the specific gastric cell line. Furthermore, of the human gastric ESTs, 766 genes (9.5%) were present as putative alternatively spliced variants. Confirmation of the predicted spliced isoforms using RT-PCR showed that the predicted isoforms exist in gastric cancer cells and some isoforms coexist in gastric cell lines. These results provide potentially useful information for elucidating the molecular mechanisms associated with gastric oncogenesis.
KeywordsGastric Cancer Transcription Start Site Gastric Cancer Cell Gastric Cancer Cell Line Human Gastric Cancer
This work was supported by a grant from the 21C Frontier Functional Human Genome Project From Ministry of Science and Technology of Korea. The authors thank Sumio Sugano, Institute of Medical Science, University of Tokyo, Japan, for technical support in the construction of the full-length cDNA library, and Marcelo Bento Soares, Iowa University, USA, for technical support in the construction of the normalized cDNA library. The authors thank Hee-Young Ahn, Ju-Yeon Lee, Mi-Young Chu, and Wan Song for their excellent technical support for EST sequencing.
- Beck AJ, Sharp PA (1977) Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell 12: 721–732Google Scholar
- Nasrin N, Ercolani L, Denaro M, Kong XF, Kang I, et al. (1990) An insulin response element in the glyceraldehyde-3–phosphate dehydrogenase gene binds a nuclear protein induced by insulin in cultured cells and by nutritional manipulations in vivo. Proc Natl Acad Sci U S A 87: 5273–5277 PubMedGoogle Scholar
- Phillips AV, Timchenko LT, Cooper TA (1998) Disruption of splicing regulated by a CUG-binding protein in myotonic dystrophy. Science 280: 737–741Google Scholar