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Mammalian Genome

, Volume 16, Issue 7, pp 505–515 | Cite as

QTL mapping for traits associated with stress neuroendocrine reactivity in rats

  • Bastien Llamas
  • Vincent Contesse
  • Véronique Guyonnet–Duperat
  • Hubert Vaudry
  • Pierre Mormède
  • Marie-Pierre MoisanEmail author
Article

Abstract

In the present study we searched for quantitative trait loci (QTLs) that affect neuroendocrine stress responses in a 20-min restraint stress paradigm using Brown–Norway (BN) and Wistar–Kyoto–Hyperactive (WKHA) rats. These strains differed in their hypothalamic–pituitary–adrenal axis (plasma ACTH and corticosterone levels, thymus, and adrenal weights) and in their renin–angiotensin–aldosterone system reactivity (plasma renin activity, aldosterone concentration). We performed a whole-genome scan on a F2 progeny derived from a WKHA × BN intercross, which led to the identification of several QTLs linked to plasma renin activity (Sr6, Sr8, Sr11, and Sr12 on chromosomes RNO2, 3, 19, and 8, respectively), plasma aldosterone concentration (Sr7 and Sr9 on RNO2 and 5, respectively), and thymus weight (Sr10, Sr13, and Srl4 on RNO5, 10, and 16, respectively). The type 1b angiotensin II receptor gene (Agtrlb) maps within the confidence intervals of QTLs on RNO2 linked to plasma renin activity (Sr6, highly significant; LOD = 5.0) and to plasma aldosterone level (Sr7, suggestive; LOD = 2.0). In vitro studies of angiotensin II–induced release of aldosterone by adrenal glomerulosa cells revealed a lower receptor potency (log EC50 = −8.16 ± 0.11 M) and efficiency (Emax = 453.3 ± 25.9 pg/3 × 104 cells/24 h) in BN than in WKHA (log EC50 = −10.66 ± 0.18 M; Emax = 573.1 ± 15.3 pg/3 × 104 cells/24 h). Moreover, differences in Agtr1b mRNA abundance and sequence reinforce the putative role of the Agtr1b gene in the differential plasma renin stress reactivity between the two rat strains.

Keywords

Quantitative Trait Locus Aldosterone Plasma Renin Activity Mineralocorticoid Receptor Suggestive Quantitative Trait Locus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • Bastien Llamas
    • 1
  • Vincent Contesse
    • 2
  • Véronique Guyonnet–Duperat
    • 1
  • Hubert Vaudry
    • 2
  • Pierre Mormède
    • 1
  • Marie-Pierre Moisan
    • 1
    Email author
  1. 1.Laboratoire Neurogénétique et StressINSERM U471/INRA UMR1243, Université Victor Segalen Bordeaux 2, Institut François MagendiecedexFrance
  2. 2.Laboratory of Cellular and Molecular NeuroendocrinologyEuropean Institute for Peptide Research, IFRMP 23, INSERM U413,UA CNRS, University of RouencedexFrance

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