Mammalian Genome

, Volume 16, Issue 8, pp 594–598 | Cite as

Mapping and expression studies of the mir17-92 cluster on pig Chromosome 11

  • Milena Sawera
  • Jan Gorodkin
  • Susanna Cirera
  • Merete FredholmEmail author


We have identified the first porcine microRNA (miRNA) cluster (the mir17-92 cluster) and localized it to the q-arm of pig Chromosome 11. The miRNA cluster was found by sequence similarity search with human miRNA sequences against the pig genomic data generated within the Sino-Danish pig genome project. The resulting data contained three complete and two incomplete miRNA precursors of seven miRNAs from the human mir17-92 cluster. Because there is a 100% sequence identity between the four pig miRNAs and the corresponding human miRNAs, the sequences of three unavailable pig miRNAs were derived from the human data. The expression profiles of seven studied miRNAs were analyzed by hybridization to Northern blots containing five porcine tissues: cerebellum, cortex, hippocampus, kidney, and liver. In order to determine the localization of the mir17-92 cluster in the pig genome, we mapped it by PCR in the porcine somatic cell hybrid (SCH) panel and in the INRA-University of Minnesota (INRA-UMN) porcine radiation hybrid (IMpRH) panel. The PCR results enabled us to localize this cluster to the q-arm of pig Chromosome 11 and map it in relation to two microsatellites. Our study presents the first expression analyses of miRNAs in pig and adds information for further functional studies in this species.


miRNA Gene Mature miRNAs Radiation Hybrid Somatic Cell Hybrid miRNA Precursor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors wish to thank Ami Klein for computational assistance and Agnieszka Zygadło and René Mikkelsen for the electroblotting equipment. This research was supported by The Danish Technical Council, the Danish Agricultural and Veterinary Research Council, The Danish Ministry of Food, Agriculture and Fisheries, and through a Ph.D. grant to M. Sawera from The Royal Veterinary and Agricultural University in Denmark.


  1. Ambros V, Bartel B, Bartel DP, Burge CB, Carrington JC, et al. (2003) A uniform system for microRNA annotation. RNA 9:277–279CrossRefPubMedGoogle Scholar
  2. Bartel DP (2004) MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell 116:281–297CrossRefPubMedGoogle Scholar
  3. Cai X, Hagedorn CH, Cullen BR (2004) Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs. RNA 10:1957–1966CrossRefPubMedGoogle Scholar
  4. Griffiths-Jones S (2004) The microRNA Registry Nucleic Acids Res 32:D109–D111CrossRefGoogle Scholar
  5. He L, Hannon GJ (2004) MicroRNAs: small RNAs with a big role in gene regulation. Nature 5:522–531Google Scholar
  6. Houbaviy HB, Murray MF, Sharp PA (2003) Embryonic stem cell-specific microRNAs. Dev Cell 5:351–358CrossRefPubMedGoogle Scholar
  7. Hutvágner G, McLachlan J, Pasquinelli AE, Balint E, Tuschl T, et al. (2001) A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA. Science 293:834–838CrossRefPubMedGoogle Scholar
  8. John B, Enright AJ, Aravin A, Tuschl T, Sander C, et al. (2004) Human microRNA targets. PLoS Biol 2:e363CrossRefPubMedGoogle Scholar
  9. Kim J, Krichevsky A, Grad Y, Hayes GD, Kosik KS, et al. (2004) Identification of many microRNAs that copurify with polyribosomes in mammalian neurons. Proc Natl Acad Sci U S A 101:360–365CrossRefPubMedGoogle Scholar
  10. Lagos-Quintana M, Rauhut R, Lendeckel W, Tuschl T (2001) Identification of novel genes coding for small expressed RNAs. Science 294:853–858CrossRefPubMedGoogle Scholar
  11. Lagos-Quintana M, Rauhut R, Yalcin A, Meyer J, Lendeckel W, et al. (2002) Identification of tissue-specific microRNAs from mouse. Curr Biol 12:735–739CrossRefPubMedGoogle Scholar
  12. Lee RC, Ambros V (2001) An extensive class of small RNAs in Caenorhabditis elegans. Science 294:862–864CrossRefPubMedGoogle Scholar
  13. Lee Y, Jeon K, Lee J-T, Kim S, Kim VN (2002) MicroRNA maturation: stepwise processing and subcellular localization. EMBO J 12:4663–4670CrossRefGoogle Scholar
  14. Lim LP, Glasner ME, Yekta S, Burge CB, Bartel DP (2003) Vertebrate microRNA genes. Science 299:1540CrossRefPubMedGoogle Scholar
  15. Mourelatos Z, Dostie J, Paushkin S, Sharma A, Charroux B, et al. (2002) miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs. Genes Dev 16:720–728CrossRefPubMedGoogle Scholar
  16. Murchison EP, Hannon GJ (2004) miRNAs on the move: miRNA biogenesis and the RNAi machinery. Curr Opin Cell Biol 16:223–229CrossRefPubMedGoogle Scholar
  17. Ota A, Tagawa H, Karnan S, Tsuzuki S, Karpas A, et al. (2004) Identification and characterization of a novel gene, C13orf25, as a target for 13q31-q32 amplification in malignant lymphoma. Cancer Res 64:3087–3095CrossRefPubMedGoogle Scholar
  18. Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol 132:356–386Google Scholar
  19. Sempere LF, Freemantle S, Pitha-Rowe I, Moss E, Dmitrovsky E, et al. (2004) Expression profiling of mammalian microRNAs uncovers a subset of brain-expressed microRNAs with possible roles in murine and human neuronal differentiation. Genome Biol 5:R13CrossRefPubMedGoogle Scholar
  20. Tanzer A, Stadler PF (2004) Molecular evolution of a microRNA cluster. J Mol Biol 339:327–335CrossRefPubMedGoogle Scholar
  21. Wernersson R, Schierup MH, Jørgensen FG, Gorodkin J, Panitz F, et al. (2005) Pigs in sequence space: A 0.66X coverage pig genome survey based on shotgun sequencing. BMC Genomics 6:70CrossRefPubMedGoogle Scholar
  22. Yerle M, Echard G, Robic A, Mairal A, Dubut-Fontana C, et al. (1996) A somatic cell hybrid panel for pig regional gene mapping characterized by molecular cytogenetics. Cytogenet Cell Genet 73:194–202PubMedGoogle Scholar
  23. Yerle M, Pinton P, Robic A, Alfonso A, Palvadeau Y, et al. (1998) Construction of a whole-genome radiation hybrid panel for high-resolution gene mapping in pigs. Cytogenet Cell Genet 82:182–188CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media Inc. 2005

Authors and Affiliations

  • Milena Sawera
    • 1
  • Jan Gorodkin
    • 1
  • Susanna Cirera
    • 1
  • Merete Fredholm
    • 1
    Email author
  1. 1.Division of Animal Genetics, Department of Animal and Veterinary Basic SciencesThe Royal Veterinary and Agricultural UniversityFrederiksberg C

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