Mammalian Genome

, Volume 15, Issue 4, pp 315–322

Characterization of 298 ESTs from porcine back fat tissue and their assignment to the SSRH radiation hybrid map

  • Ayumi Mikawa
  • Hideaki Suzuki
  • Kohei Suzuki
  • Daisuke Toki
  • Hirohide Uenishi
  • Takashi Awata
  • Noriyuki Hamasima
Article

Abstract

Following several criteria, we collected, clustered, and functionally categorized 653 expressed sequence tags (ESTs) of 5′ ends from porcine back fat libraries from the >15,000 porcine ESTs collected to date. By searching the LocusLink and Mapviewer database, we knew the positions of these 653 ESTs on human chromosomes (HSAs). Sus scrofa radiation hybrid (SSRH) mapping revealed that 298 porcine EST clusters out of 653 were localized near microsatellite (MS) markers. Among these EST clusters, we could assign 182 to their porcine chromosomes (SSCs) on the SSRH map.

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Copyright information

© Springer-Verlag New York Inc. 2004

Authors and Affiliations

  • Ayumi Mikawa
    • 1
  • Hideaki Suzuki
    • 1
  • Kohei Suzuki
    • 1
  • Daisuke Toki
    • 1
  • Hirohide Uenishi
    • 2
  • Takashi Awata
    • 2
  • Noriyuki Hamasima
    • 2
  1. 1.Animal Genome Research Program Team (AGP), Society for Techno-Innovation of AgricultureForestry and Fisheries (STAFF) Institute, Tsukuba, Ibaraki 305-0854Japan
  2. 2.Animal Genome Laboratory (AGP), Genome Research Department, Genome and Biodiversity Research CenterNational Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602Japan

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