Mammalian Genome

, Volume 15, Issue 2, pp 142–148

Genotype of stearoyl-CoA desaturase is associated with fatty acid composition in Japanese Black cattle

  • Masaaki Taniguchi
  • Takeshi Utsugi
  • Kenji Oyama
  • Hideyuki Mannen
  • Masato Kobayashi
  • Yoshihiro Tanabe
  • Atsushi Ogino
  • Soichi Tsuji


To investigate the genetic factors that affect fatty acid composition of beef, we compared the full-length bovine stearoyl-CoA desaturase (SCD) cDNA from 20 Japanese Black steers. Two types of the SCD gene with single nucleotide polymorphisms (SNPs) were observed in the ORF of SCD cDNA, in which an amino acid replacement from valine (type V) to alanine (type A) was predicted. We developed a method for genotyping these two SCD genes based on PCR-RFLP. We have classified 1003 Japanese Black carcasses into three genotypes, VV, VA, and AA, and compared fatty acid composition among them. The SCD type A gene contributed to higher MUFA percentage and lower melting point in intramuscular fat. The SCD genotype was not the only genetic factor contributing to fatty acid composition of Japanese Black steers, but the SCD genotype was considered one of the causes of genetic variation in fatty acid composition of Japanese Black steers. Transcription factors such as sterol regulatory element binding protein-1c (SREBP-1c) may account for the remaining part of the genetic variation in fatty acid composition.


  1. 1.
    Bene, H, Lasky, D, Ntambi, JM 2001Cloning and characterization of the human stearoyl-CoA desaturase gene promoter: transcriptional activity by sterol regulatory elementbinding protein and repression by polyunsaturated fatty acids and cholesterol.Biochem Biophys Res Commun28411941198CrossRefPubMedGoogle Scholar
  2. 2.
    Bernard, L, Leroux, C, Hayes, H, Gautier, M, Chilliard, Y,  et al. 2001Characterization of the caprine stearoyl-CoA desaturase gene and its mRNA showing an unusually long 3′-UTR sequence arising from a single exon.Gene2815361CrossRefPubMedGoogle Scholar
  3. 3.
    Cho, HP, Nakamura, MT, Clarke, SD 1999Cloning, expression, and nutritional regulation of the mammalian Δ6 desaturase.J Biol Chem274471477CrossRefPubMedGoogle Scholar
  4. 4.
    Chung, M, Ha, S, Jeong, S, Bok, J, Cho, K, Baik, M, Choi, Y 2000Cloning and characterization of bovine stearoyl CoA desaturase 1 cDNA from adipose tissues.Biosci Biotechnol Biochem6415261530PubMedGoogle Scholar
  5. 5.
    Clemens, E, Arthaud, V, Mandigo, R, Woods, W 1973Fatty acid composition of bulls and steers as influenced by age and dietary energy level.J Anim Sci3713261331PubMedGoogle Scholar
  6. 6.
    Falconer, DS 1981Introduction to Quantitative Genetics, 2nd ed.Longman Inc.New York100111Google Scholar
  7. 7.
    Folch, J, Lees, M, Stanley, GHS 1957A simple method for the isolation and purification of total lipids from animal tissues.J Biol Chem226487509Google Scholar
  8. 8.
    Haliassos, A, Chomel, JC, Grandjouan, S, Kruh, J, Kaplan, JC,  et al. 1989Detection of minority point mutations by modified PCR technique: a new approach for a sensitive diagnosis of tumor-progression markers.Nucleic Acids Res1780938099PubMedGoogle Scholar
  9. 9.
    Kim, YC, Ntambi, JM 1999Regulation of stearoyl-CoA desaturase genes: role in cellular metabolism and preadipocyte differentiation.Biochem Biophys Res Commun26614CrossRefPubMedGoogle Scholar
  10. 10.
    Kumar, S, Tamura, K, Jakobsen, IB, Nei, M 2001MEGA2: Molecular evolutionary genetics analysis software.Bioinformatics1712441245PubMedGoogle Scholar
  11. 11.
    Mandell, IB, Buchanan-Smith, JG, Campbell, CP 1998Effects of forage vs grain feeding on carcass characteristics, fatty acid composition, and beef quality in Limousin-cross steers when time on feed is controlled.J Anim Sci7626192630PubMedGoogle Scholar
  12. 12.
    Melton, SL, Amiri, M, Davis, GW, Backus, WR 1982Flavor and chemical characteristics of ground beef from grass-, forage-, grain- and grain-finished steers.J Anim Sci557787Google Scholar
  13. 13.
    Ntambi, JM, Buhrow, SA, Kaestner, KH, Christy, RJ, Sibley, E,  et al. 1988Differentiation-induced gene expression in 3T3-L1 preadipocytes.J Biol Chem2631729117300PubMedGoogle Scholar
  14. 14.
    Oikawa, T, Sanehira, T, Sato, K, Mizoguchi, Y, Yamamoto, H,  et al. 2000Genetic parameters for growth and carcass traits of Japanese Black (Wagyu) cattle.Anim Sci715964Google Scholar
  15. 15.
    Oka, A, Iwaki, F, Dohgo, T, Ohtagaki, S, Noda, M,  et al. 2002Genetic effects on fatty acid composition of carcass fat of Japanese Black Wagyu steers.J Anim Sci8010051011PubMedGoogle Scholar
  16. 16.
    O’Keefe, PW, Wellington, GH, Mattick, LR, Stouffer, JR 1968Composition of bovine muscle lipids at various carcass locations.J Food Sci33188192Google Scholar
  17. 17.
    Perry, D, Nicolls, PJ, Thompson, JM 1998The effect of sire breed on the melting point and fatty acid composition of subcutaneous fat in steers.J Anim Sci768795PubMedGoogle Scholar
  18. 18.
    Sessler, AM, Kaur, N, Palta, JP, Ntambi, JM 1996Regulation of stearoyl-CoA desaturase 1 mRNA stability by polyunsaturated fatty acid in 3T3-L1 adipocytes.J Biol Chem2712985429858CrossRefPubMedGoogle Scholar
  19. 19.
    Shanklin, J, Whittle, E, Fox, BG 1994Eight histidine residues are catalytically essential in a membrane-associated iron enzyme, stearoyl-CoA desaturase, and are conserved in alkane hydroxyrase and xylene monooxygenase.Biochemistry331278712794PubMedGoogle Scholar
  20. 20.
    Smith, SB, Yang, A, Larsen, TW, Tume, RK 1998Positional analysis of triacylglycerols from bovine adipose tissue lipids varying in degree of unsaturation.Lipids33197207PubMedGoogle Scholar
  21. 21.
    Strittmatter, P, Thiede, M, Hackett, CS, Ozols, J 1988Bacterial synthesis of active rat stearyl-CoA desaturase lacking the 26-residue amino-terminal amino acid sequence.J Biol Chem26325322535PubMedGoogle Scholar
  22. 22.
    Taniguchi, M, Mannen, H, Oyama, K, Shimakura, Y, Watanabe, H,  et al. 2004Differences in stearoyl-CoA desaturase mRNA levels between Japanese Black and Holstein cattle.Livestock Prod Sci (in press)..Google Scholar
  23. 23.
    Ward, RJ, Travers, MT, Richards, SE, Vernon, RG, Salter, AM,  et al. 1998Stearoyl-CoA desaturase mRNA is transcribed from a single gene in the ovine genome.Biochim Biophys Acta1391145156CrossRefPubMedGoogle Scholar
  24. 24.
    Yang, A, Larsen, TW, Powell, VH, Tume, RK 1999aA comparison of fat composition of Japanese and long-term grain-fed Australian steers.Meat Sci5119Google Scholar
  25. 25.
    Yang, A, Larsen, TW, Smith, SB, Tume, RK 1999bΔ9 desaturase activity in bovine subcutaneous adipose tissue of different fatty acid composition.Lipids34971978Google Scholar
  26. 26.
    Yoshimura, T, Namikawa, K 1985Influence of breed, sex and anatomical location on lipid and fatty acid composition of bovine subcutaneous fat.Jpn J Zootech Sci56130136Google Scholar
  27. 27.
    Zembayashi, M, Nishimura, K, Lunt, DK, Smith, SB 1995Effect of breed type and sex on the fatty acid composition of subcutaneous and intramuscular lipids of finishing steers and heifers.J Anim Sci7333253332PubMedGoogle Scholar
  28. 28.
    Zhang, L, Parismoo, LG, Stenn, K, Prouty, SM 1999Human stearoyl-CoA desaturase: alternative transcripts generated from a single gene by usage of tandem polyadenylation sites.Biochem J340255264CrossRefPubMedGoogle Scholar
  29. 29.
    Zhang, L, Ge, L, Tran, T, Stenn, K, Prouty, SM 2001Isolation and characterization of the human stearoyl-CoA desaturase gene promoter: requirement of a conserved CCAAT cis element.Biochem J357183193CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 2004

Authors and Affiliations

  • Masaaki Taniguchi
    • 1
  • Takeshi Utsugi
    • 1
  • Kenji Oyama
    • 2
  • Hideyuki Mannen
    • 2
  • Masato Kobayashi
    • 3
  • Yoshihiro Tanabe
    • 4
  • Atsushi Ogino
    • 4
  • Soichi Tsuji
    • 2
  1. 1.Laboratory of Animal Breeding and GeneticsGraduate School of Science and Technology, Kobe University, Nada-ku, Kobe 657-8501Japan
  2. 2.Laboratory of Animal Breeding and GeneticsFaculty of Agriculture, Kobe University, Nada-ku, Kobe 657-8501Japan
  3. 3.Yamagata Agricultural Research and Training Center, Shinzyo, 996-0041Japan
  4. 4.Maebashi Institute of Animal ScienceLivestock Improvement Association of Japan Inc., Maebashi 371-0121nJapan

Personalised recommendations