Mammalian Genome

, Volume 14, Issue 10, pp 692–702 | Cite as

The mapping of quantitative trait loci underlying strain differences in locomotor activity between 129S6 and C57BL/6J mice

  • Michele A. Kelly
  • Malcolm J. Low
  • Tamara J. Phillips
  • Edward K. Wakeland
  • Masashi Yanagisawa
Article

Abstract

Performance in the open field and rotarod paradigms, two common assessments of locomotor function, have been demonstrated to be strain dependent in mice. In this study, eight significant quantitative trait loci (QTL) for behavior phenotypes in either the open field or rotarod paradigms were identified between the 129S6 (129/SvEvTac) and C57BL/6J strains. These strains were chosen for comparison because of their frequent use in the generation of mutant mice from gene-targeted, embryonic stem cells. Two of the QTLs for horizontal distance traveled are located on Chromosomes (Chrs) 1 and 12 and closely replicate the findings of other groups using different strains of mice. Rotarod performance was influenced in an oppositional manner by two separate QTLs on Chr 1 and 2. Additionally, examination of several different aspects of behavior in the open field revealed significant QTLs for average speed (Chr 12), duration (Chrs 2, 16, and 18), time spent in motion (Chr 16), vertical movements (rearing) (Chrs 6 and 12), and vertical time (rearing time) (Chrs 6 and 12). Our finding of independent QTLs for these topographic components of open field activity supports the idea that they are separate and distinct from total horizontal distance traveled and should be studied independently. The QTLs described in this study, in combination with our panel of polymorphic chromosomal markers for 129S6 and C57BL/6J strains, will be useful in assessing the potential epistatic effects of parental strain background on the phenotypes of genetically modified mice.

Notes

Acknowledgements

The authors thank the following institutions for support: Department of Veterans Affairs (T.J. Phillips), NIDA R01 grant DA10913 (T.J. Phillips), NIAAA Alcohol Center grant AA10760 (T.J. Phillips), NIDA training grant T32 DA07262 (M.A. Kelly), NIDDK training grant T32DK07257 (M.A. Kelly), and funding from ERATO/JST (M. Yanagisawa). M. Yanagisawa is an Investigator and M.A. Kelly is an Associate of the Howard Hughes Medical Insitute.

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Copyright information

© Springer-Verlag New York Inc. 2003

Authors and Affiliations

  • Michele A. Kelly
    • 1
  • Malcolm J. Low
    • 2
    • 3
  • Tamara J. Phillips
    • 3
    • 4
  • Edward K. Wakeland
    • 5
  • Masashi Yanagisawa
    • 1
    • 6
  1. 1.HHMI and Dept. Molecular GeneticsThe University of Texas, Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390-9050USA
  2. 2.Vollum InstituteOregon Health and Science University, Portland, OregonUSA
  3. 3.Dept. Behavioral NeuroscienceOregon Health and Science University, Portland, OregonUSA
  4. 4.The Veterans Affairs Medical Center, Portland, OregonUSA
  5. 5.Center for ImmunologyThe University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390USA
  6. 6.ERATO Yanagisawa Orphan Receptor ProjectJapan Science and Technology Corporation, TokyoJapan

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