Mammalian Genome

, Volume 13, Issue 10, pp 578–587 | Cite as

A first-generation EST RH comparative map of the porcine and human genome

  • Anette  Rink
  • Elizabeth M. Santschi
  • Katie M. Eyer
  • Benjamin  Roelofs
  • Markus  Hess
  • Myra  Godfrey
  • Elif K. Karajusuf
  • Martine  Yerle
  • Denis  Milan
  • Craig W. Beattie

We have constructed a first-generation EST radiation hybrid comparative map of the porcine genome by assigning 1058 markers to the IMpRH7000 panel. Chromosomal localization was determined with a 2pt LOD of 4.8 for 984 markers, using the IMpRH mapping tool. Annotated ESTs represent 46.2% or 489 of the markers. Marker distribution was not stochastic and ranged from 0.41 for SSC8 to 1.77 for SSC12, respectively. Two hundred fifty-one markers assigned to the physical map of the pig did not find a homologous sequence in V22 of the human genome assembly, indicative of gaps in the assembled human genome sequence. The comparative porcine/human map covers 3290 MB, or 98.3% of the presumed size of the human genome. However, 60 breakpoints were identified between chromosomes, as well as 90 micro-rearrangements within synteny groups. Six porcine chromosomes—SSC2, 5, 6, 7, 12, and 14—correspond to the three gene-richest human chromosomes, HSA17, 19, and 22, and show above average marker density. Porcine Chrs 1, 8, 11, and X display a low DNA/marker ratio and correspond to the 'genome deserts' on HSA 18, 4, 13, and X.

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Copyright information

© Springer-Verlag New York Inc. 2002

Authors and Affiliations

  • Anette  Rink
    • 1
  • Elizabeth M. Santschi
    • 2
  • Katie M. Eyer
    • 1
  • Benjamin  Roelofs
    • 1
  • Markus  Hess
    • 1
  • Myra  Godfrey
    • 1
  • Elif K. Karajusuf
    • 1
  • Martine  Yerle
    • 3
  • Denis  Milan
    • 3
  • Craig W. Beattie
    • 1
  1. 1.Department of Animal Biotechnology, College of Agriculture, Biotechnology and Natural Resources, University of Nevada, Reno, Nevada 89557, USAUS
  2. 2.Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, Wisconsin 53706, USAUS
  3. 3.Institut National de la Recherche Agronomique, Laboratoire de Genetique Cellulaire, Castanet-Tolosan, FranceFR

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