Mammalian Genome

, Volume 12, Issue 10, pp 787–792 | Cite as

Downregulation of Tfam and mtDNA copy number during mammalian spermatogenesis

  • Anja Rantanen
  • Monica Jansson
  • Anders Oldfors
  • Nils-Göran Larsson
Article

Abstract.

Mitochondrial transcription factor A (Tfam) is required for mtDNA maintenance, and mitochondrial Tfam protein levels directly affect mtDNA copy number. Previous studies have shown significant reduction of Tfam protein levels in mitochondria together with the appearance of abundant testis-specific Tfam mRNA isoforms as spermatogenesis proceeds in both mouse and man. Interestingly, an abundant testis-specific nuclear Tfam protein isoform of unknown function is found in the mouse, but not in humans. We have now characterized Tfam expression in rat testis to identify conserved features in mammalian spermatogenesis. The nuclear Tfam protein isoform is absent in the rat and is thus dispensable for mammalian spermatogenesis. Similar to mice and humans, we found expression of alternate Tfam transcripts, downregulation of mitochondrial Tfam protein levels, and downregulation of mtDNA copy number during rat spermatogenesis. These features are thus common to all mammals and may provide one of several mechanisms preventing paternal mtDNA transmission.

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Copyright information

© Springer-Verlag New York Inc. 2001

Authors and Affiliations

  • Anja Rantanen
    • 1
  • Monica Jansson
    • 2
  • Anders Oldfors
    • 2
  • Nils-Göran Larsson
    • 1
  1. 1.Department of Medical Nutrition, Karolinska Institutet, Novum, Huddinge Hospital, S-14186 Huddinge, 76 Stockholm, SwedenSweden
  2. 2.Department of Pathology, Sahlgranska Hospital, S-413 45 Göteborg, SwedenSweden

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