Better understanding of acute gouty attack using CT perfusion in a rabbit model
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To assess hemodynamic changes related to acute gouty knee arthritis in a rabbit with CT perfusion (CTP)
Forty-two rabbits were randomly separated into two groups: the treated group of 30 and the control group of 12. The right knee was injected with monosodium urate solution and polymyxin in the treated group and saline and polymyxin in the control group. At 2, 16, 32, 48, 60, and 72 h after injection, five rabbits from the treated group and two rabbits from the control group were selected for CTP. At each time point, blood flow (BF), blood volume (BV), and clearance rate (CL) were measured, and microvessel density (MVD) was evaluated with a microscope.
In the treated group, BF, BV, CL, and MVD were significantly higher than in the control group (p < 0.001). Differences within paired comparison of BV, BF, CL, and MVD were all significant (all p < 0.001). Peak time of BV, BF, and MVD was 32 h and 48 h for CL. After multivariate stepwise linear regression analysis, BV was linearly associated with MVD and vice versa, which also applied to BF with MVD and BF with CL, separately. The ascending rate of MVD was the highest among that of all parameters; so was the descending rate of CL.
CTP in this rabbit knee model accurately detected hemodynamic changes during a gouty attack.
• Acute gouty arthritis can be evaluated with CTP in a rabbit knee model.
• Following injection of MSU crystals, producing an acute gouty attack, CTP successfully assessed hemodynamic changes.
• The ascending rate of MVD was the highest among that of all parameters; so was the descending rate of CL.
KeywordsPerfusion imaging Multidetector computed tomography Arthritis, gouty Rabbits
Computed tomography perfusion
Intraclass correlation coefficient
This study has received funding from The science and technology development of Shandong province (2011GSF11834).
Compliance with ethical standards
The scientific guarantor of this publication is Qing Yang.
Conflict of interest
The authors declare that they have no competing interests.
Statistics and biometry
No complex statistical methods were necessary for this paper.
Approval from the institutional animal care committee was obtained.
Institutional Review Board approval was obtained.
• Randomized controlled trial/experimental
• Performed at one institution
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