European Radiology

, Volume 28, Issue 5, pp 2176–2183 | Cite as

Hemispherical photoacoustic imaging of myocardial infarction: in vivo detection and monitoring

  • Jing Lv
  • Ya Peng
  • Shi Li
  • Zhide Guo
  • Qingliang Zhao
  • Xianzhong Zhang
  • Liming Nie
Cardiac

Abstract

Objectives

This study aimed to demonstrate the capacity for noninvasive localisation and characterisation of myocardial infarction (MI) in vivo using a hemispherical photoacoustic imaging (PAI) system. MI remains a leading cause of morbidity and mortality worldwide. To enable optimal treatment of patients, timely and accurate diagnosis and longitudinal monitoring is critical.

Methods

Ischaemia was induced in Balb/c mice by ligation of the left anterior descending artery. The hemispherical PAI system, equipped with 128 ultrasonic transducers spirally distributed on the surface, along with parallel data acquisition, was applied for imaging of the mouse heart.

Results

Our study showed that hemispherical PAI can delineate thoracic vessels and the morphology of the entire heart. Longitudinal PAI images revealed gradual expansion of the infarcted area along with necrosis and fibrosis, which were quantitatively validated by triphenyltetrazolium chloride staining. After MI modelling, the photoacoustic (PA) signal intensity decreased by 399.1 ± 56.3 (p < 0.001), a ~2.5-fold reduction compared to that of healthy cardiac tissue. The calculated size of the enlarged heart, 10.4 ± 6.0 mm2 (p < 0.001), represents an increase of ~18% versus that of a healthy heart.

Conclusions

PAI enables MI diagnosis and injury localisation with its capabilities for both deep organ imaging and lesion region differentiation.

Key Points

Photoacoustic imaging (PAI), combining optical absorption and ultrasonic resolution, can delineate cardiac anatomy.

PAI can diagnose myocardial infarction lesions with 10 mm imaging depth in vivo.

Quantified results are in excellent agreement with enzyme and histological examinations.

PAI can serve as a complementary modality to SPECT and ultrasound imaging.

This study will encourage further PAI development for clinical use.

Keywords

Myocardial infarction Optical imaging Diagnosis Ultrasonography Single-photon emission computed tomography 

Abbreviations

MI

Myocardial infarction

SPECT

Single-photon emission computed tomography

US

Ultrasound

PA

Photoacoustic

PAI

Photoacoustic imaging

ROI

Region of interest

sO2

Oxygen saturation

cTnT

Cardiac troponin T

TTC

Triphenyltetrazolium chloride

LVIDd

Left ventricular internal diameter diastole

LVIDs

Left ventricular internal diameter systole

LVEF

Left ventricular ejection fraction

LVFS

Left ventricular fraction shortening

Notes

Compliance with ethical standards

Guarantor

The scientific guarantor of this publication is Liming Nie, PhD.

Conflict of interest

The authors of this manuscript declare no relationships with any companies whose products or services may be related to the subject matter of the article.

Statistics and biometry

Yeda Chen kindly provided statistical advice for this manuscript. No complex statistical methods were necessary for this paper.

Ethical approval

Approval from the institutional animal care committee was obtained.

Methodology

• Prospective

• Experimental

• Performed at one institution

Supplementary material

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ESM 1 (DOCX 2243 kb)
330_2017_5209_MOESM2_ESM.mov (11.4 mb)
ESM 2 (MOV 11657 kb)
330_2017_5209_MOESM3_ESM.mov (10.7 mb)
ESM 3 (MOV 10971 kb)

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Copyright information

© European Society of Radiology 2017

Authors and Affiliations

  • Jing Lv
    • 1
  • Ya Peng
    • 1
  • Shi Li
    • 1
  • Zhide Guo
    • 1
  • Qingliang Zhao
    • 1
  • Xianzhong Zhang
    • 1
  • Liming Nie
    • 1
  1. 1.State Key Laboratory of Molecular Vaccinology and Molecular Diagnosis & Center for Molecular Imaging and Translational Medicine, School of Public HealthXiamen UniversityXiamenPeople’s Republic of China

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