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European Radiology

, Volume 28, Issue 1, pp 363–371 | Cite as

High resolution post-mortem MRI of non-fixed in situ foetal brain in the second trimester of gestation: Normal foetal brain development

  • Elisa ScolaEmail author
  • Giorgio Conte
  • Giovanni Palumbo
  • Sabrina Avignone
  • Claudia Maria Cinnante
  • Simona Boito
  • Nicola Persico
  • Tommaso Rizzuti
  • Fabio Triulzi
Neuro

Abstract

Purpose

To describe normal foetal brain development with high resolution post-mortem MRI (PMMRI) of non-fixed foetal brains.

Methods

We retrospectively collected PMMRIs of foetuses without intracranial abnormalities and chromosomal aberrations studied after a termination of pregnancy due to extracranial abnormalities or after a spontaneous intrauterine death. PMMRIs were performed on a 3-T scanner without any fixation and without removing the brain from the skull. All PMMRIs were evaluated in consensus by two neuroradiologists.

Results

Our analysis included ten PMMRIs (median gestational age (GA): 21 weeks; range: 17–28 weeks). At 19 and 20 weeks of GA, the corticospinal tracts are recognisable in the medulla oblongata, becoming less visible from 21 weeks. Prior to 20 weeks the posterior limb of the internal capsule (PLIC) is more hypointense than surrounding deep grey nuclei; starting from 21 weeks the PLIC becomes isointense, and is hyperintense at 28 weeks. From 19–22 weeks, the cerebral hemispheres show transient layers: marginal zone, cortical plate, subplate, and intermediate, subventricular and germinal zones.

Conclusion

PMMRI of non-fixed in situ foetal brains preserves the natural tissue contrast and skull integrity. We assessed foetal brain development in a small cohort of foetuses, focusing on 19–22 weeks of gestation.

Key Points

Post-mortem magnetic resonance imaging (PMMRI) of non-fixed head is feasible.

PMMRI of unfixed in situ foetal brains preserves the natural tissue contrast.

PMMRI provide a good depiction of the normal foetal brain development.

PMMRI of unfixed in situ foetal brains preserves the skull integrity.

PMMRI pattern of foetal brain development at early gestational age is described.

Keywords

Magnetic resonance imaging Brain Post-mortem Autopsy Foetus 

Abbreviations

CP

Cortical plate

GA

Gestational age

GZ

Germinal zone

IZ

Intermediate zone and subventricular zone

MRI

Magnetic resonance imaging

MZ

Marginal zone

PMMRI

Post-mortem magnetic resonance imaging

SP

Subplate

TOP

Termination of pregnancy

Notes

Compliance with ethical standards

Guarantor

The scientific guarantor of this publication is Prof. Fabio Triulzi.

Conflict of interest

The authors of this manuscript declare no relationships with any companies whose products or services may be related to the subject matter of the article.

Funding

The authors state that this work has not received any funding.

Statistics and biometry

No complex statistical methods were necessary for this paper.

Informed consent

Written informed consent was waived by the Institutional Review Board.

Ethical approval

Institutional Review Board approval was obtained

Methodology

• retrospective

• observational study

• performed at one institution

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Copyright information

© European Society of Radiology 2017

Authors and Affiliations

  1. 1.Neuroradiology UnitFondazione IRCCS Ca Granda Ospedale Maggiore PoliclinicoMilanItaly
  2. 2.Postgraduation School in RadiodiagnosticsUniversità degli Studi di MilanoMilanItaly
  3. 3.Department of Obstetrics and Gynaecology ‘L. Mangiagalli’Fondazione IRCCS Ca Granda Ospedale Maggiore PoliclinicoMilanItaly
  4. 4.Pathology UnitFondazione IRCCS Ca Granda Ospedale Maggiore PoliclinicoMilanItaly
  5. 5.Department of Pathophysiology and TransplantationUniversità degli Studi di MilanoMilanItaly

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