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European Radiology

, Volume 25, Issue 6, pp 1814–1822 | Cite as

Feasibility study of novel endoscopic Cerenkov luminescence imaging system in detecting and quantifying gastrointestinal disease: first human results

  • Hao Hu
  • Xin Cao
  • Fei Kang
  • Min Wang
  • Yenan Lin
  • Muhan Liu
  • Shujun Li
  • Liping Yao
  • Jie Liang
  • Jimin Liang
  • Yongzhan Nie
  • Xueli Chen
  • Jing Wang
  • Kaichun Wu
Molecular Imaging

Abstract

Objectives

Cerenkov luminescence imaging (CLI) provides potential to use clinical radiotracers for optical imaging. The goal of this study was to present a newly developed endoscopic CLI (ECLI) system and illustrate its feasibility and potential in distinguishing and quantifying cancerous lesions of the GI tract.

Methods

The ECLI system was established by integrating an electron-multiplying charge-coupled device camera with a flexible fibre endoscope. Phantom experiments and animal studies were conducted to test and illustrate the system in detecting and quantifying the presence of radionuclide in vitro and in vivo. A pilot clinical study was performed to evaluate our system in clinical settings.

Results

Phantom and mice experiments demonstrated its ability to acquire both the luminescent and photographic images with high accuracy. Linear quantitative relationships were also obtained when comparing the ECLI radiance with the radiotracer activity (r 2 = 0.9779) and traditional CLI values (r 2 = 0.9025). Imaging of patients revealed the potential of ECLI in the identification and quantification of cancerous tissue from normal, which showed good consistence with the clinical PET examination.

Conclusions

The new ECLI system shows good consistence with the clinical PET examination and has great potential for clinical translation and in aiding detection of the GI tract disease.

Key Points

CLI preserves the characteristics of both optical and radionuclide imaging.

CLI provides great potential for clinical translation of optical imaging.

The newly developed endoscopic CLI (ECLI) has quantification and imaging capacities.

GI tract has accessible open surfaces, making ECLI a potentially suitable technique.

Cerenkov endoscopy has great clinical potential in detecting GI disease.

Keywords

Molecular imaging Radionuclide imaging Optical imaging Endoscopy Gastrointestinal disease 

Abbreviations

CLI

Cerenkov luminescence imaging

CLT

Cerenkov luminescence tomography

ECLI

Endoscopic Cerenkov luminescence imaging

EMCCD

Electron-multiplying charge-coupled device

GI

Gastrointestinal

Notes

Acknowledgments

The scientific guarantor of this publication is Xueli Chen at Xidian University. The authors would like to thank Dr. Xiaowei Ma from Department of Nuclear Medicine, Xijing Hospital, Fourth Military Medical University for the technical assistance. The authors of this manuscript declare no relationships with any companies whose products or services may be related to the subject matter of the article. This work was supported in part by the National Natural Science Foundation of China under Grant Nos. 81090270, 81090272, 81090273, 81101083, 81230033, 81371615 and 81370585; Program of the National Basic Research and Development Program of China (973) under Grant Nos. 2011CB707702, 2010CB529302, the National Municipal Science and Technology Project under Grant Nos. 2009ZX09103-667, 2009ZX09301-009-RC06, and the Open Research Project under Grant 20120101 from SKLMCCS. No complex statistical methods were necessary for this paper. Institutional review board approval was obtained. Written informed consent was obtained from all subjects (patients) in this study. Approval from the institutional animal care committee was obtained. Methodology: experimental, performed at one institution.

Supplementary material

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Supplementary Figure S1

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High resolution image (TIFF 1329 kb)
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Supplementary Figure S2

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High resolution image (TIFF 132 kb)
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Supplementary Figure S3

(GIF 32 kb)

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High resolution image (TIFF 404 kb)
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Supplementary Table S1 (DOCX 16 kb)
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ESM 1 (DOCX 18 kb)

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Copyright information

© European Society of Radiology 2015

Authors and Affiliations

  • Hao Hu
    • 1
  • Xin Cao
    • 2
  • Fei Kang
    • 3
  • Min Wang
    • 4
  • Yenan Lin
    • 2
  • Muhan Liu
    • 2
  • Shujun Li
    • 1
  • Liping Yao
    • 1
  • Jie Liang
    • 1
  • Jimin Liang
    • 2
  • Yongzhan Nie
    • 1
  • Xueli Chen
    • 2
  • Jing Wang
    • 3
  • Kaichun Wu
    • 1
  1. 1.State Key Laboratory of Cancer Biology, Department of Digestive Diseases, Xijing HospitalFourth Military Medical UniversityXi’anChina
  2. 2.School of Life Science and TechnologyXidian UniversityXi’anChina
  3. 3.Department of Nuclear Medicine, Xijing HospitalFourth Military Medical UniversityXi’anChina
  4. 4.Department of GastroenterologyXi’an Children’s HospitalXi’anChina

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