European Radiology

, Volume 23, Issue 10, pp 2830–2837 | Cite as

Efficacy of intra-tendinous injection of platelet-rich plasma in treating tendinosis: comprehensive assessment of a rat model

  • Benjamin Dallaudière
  • Marta Lempicki
  • Lionel Pesquer
  • Liliane Louedec
  • Pierre Marie Preux
  • Philippe Meyer
  • Vincent Hummel
  • Ahmed Larbi
  • Lydia Deschamps
  • Clement Journe
  • Agathe Hess
  • Alain Silvestre
  • Paul Sargos
  • Philippe Loriaut
  • Patrick Boyer
  • Elisabeth Schouman-Claeys
  • Jean Baptiste Michel
  • Jean Michel Serfaty



To assess the potential of intra-tendinous injection of platelet rich plasma (PRP) to treat tendinosis (T+) in a rat model of patellar and Achilles T+, and evaluate its local toxicity.


Thirty rats (120 patellar and Achilles tendons) were used. We induced T+ into 80 tendons (patellar = 40, Achilles = 40) by injecting collagenase at day 0 under ultrasound (US) guidance. Clinical examination and US at day 3, followed by US-guided intra-tendinous injection of either PRP (PRPT+, n = 40) or physiological serum (ST+, n = 40, control). Follow-up was at days 6, 13, 18 and 25 using clinical, US and histological evaluation. To study PRP toxicity, we injected PRP into 40 normal tendons (PRPT-) and compared with 40 untreated normal tendons (T-).


All PRPT+ showed better joint mobilisation compared with ST+ at day 6 (P = 0.005), day 13 (P = 0.02), day 18 (P = 0.003) and day 25 (P = 0.01). Similar results were found regarding US and histology, with smaller collagen fibre diameters (day 6, P = 0.003, day 25, P ≤ 0.004), less disorganisation and fewer neovessels (day 6, P = 0.003, day 25, P = 0.0003) in PRPT+ compared with ST+. Comparison between PRPT- and T- showed no PRP toxicity (P = 0.18).


Our study suggests that mono-injection of PRP in T+ improves tendon healing, with no local toxicity.

Key Points

• We assessed the potential of platelet rich plasma (PRP) to treat tendinosis.

• We treated patellar and Achilles tendinosis in a rat model.

• We evaluated clinical, imaging and histological data.

• Intra-tendinous PRP injection could be useful in the treatment of tendinosis.


Tendinosis Rat Platelet PRP US 



platelet-rich plasma


T+ tendon injected with PRP


T- tendon injected with PRP


physiological serum


T+ tendon injected with PS


tendon with tendinosis


untreated normal tendons



We wish to thank Anne Perozzielo MD ( Unité de Recherche Clinique, Paris Nord, France) for her contribution to this work.


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Copyright information

© European Society of Radiology 2013

Authors and Affiliations

  • Benjamin Dallaudière
    • 1
    • 2
    • 3
    • 9
  • Marta Lempicki
    • 1
    • 3
  • Lionel Pesquer
    • 4
  • Liliane Louedec
    • 2
  • Pierre Marie Preux
    • 5
  • Philippe Meyer
    • 4
  • Vincent Hummel
    • 1
  • Ahmed Larbi
    • 1
  • Lydia Deschamps
    • 6
  • Clement Journe
    • 7
  • Agathe Hess
    • 1
    • 3
  • Alain Silvestre
    • 4
  • Paul Sargos
    • 1
  • Philippe Loriaut
    • 8
  • Patrick Boyer
    • 8
  • Elisabeth Schouman-Claeys
    • 1
    • 3
  • Jean Baptiste Michel
    • 2
  • Jean Michel Serfaty
    • 1
    • 2
    • 3
  1. 1.Service de RadiologieHôpital universitaire BichatParisFrance
  2. 2.Inserm U698Hôpital universitaire BichatParisFrance
  3. 3.Université de Médecine Paris DiderotParisFrance
  4. 4.Centre d’imagerie ostéo articulaire, Clinique du sportBordeaux-MérignacFrance
  5. 5.Laboratoire de Biostatistiques, Faculté de médecineLimogesFrance
  6. 6.Service d’ AnatomopathologieHôpital universitaire BichatParisFrance
  7. 7.Institut Claude Bernard: Centre d’imagerie fonctionnelleParisFrance
  8. 8.Service d’ OrthopédieHôpital universitaire BichatParisFrance
  9. 9.Bichat-Claude Bernard HospitalParisFrance

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