European Radiology

, Volume 23, Issue 2, pp 375–380

The delayed effects of irreversible electroporation ablation on nerves

  • Helmut Schoellnast
  • Sebastien Monette
  • Paula C. Ezell
  • Majid Maybody
  • Joseph P. Erinjeri
  • Michael D. Stubblefield
  • Gordon Single
  • Stephen B. Solomon
Experimental

Abstract

Objective

To evaluate the delayed effects of irreversible electroporation (IRE) ablation on nerves.

Methods

The study was approved by the institutional animal care and use committee. CT-guided IRE-ablation (electric field per distance, 1,500 V/cm; pulse length, 70 μs; number of pulses, 90) of 6 sciatic nerves was performed in 6 pigs that were euthanized 2 months after ablation. The sciatic nerves were harvested immediately after euthanasia for histopathological evaluation. Sections from selected specimens were stained with haematoxylin and eosin (H&E), Masson’s trichrome (MT) method for collagen, and immunohistochemistry was performed for S100 and neurofilaments (markers for Schwann cells and axons, respectively).

Results

All nerves showed a preserved endoneural architecture and presence of numerous small calibre axons associated with Schwann cell hyperplasia, consistent with axonal regeneration. A fibrous scar was observed in the adjacent muscle tissue, confirming ablation at the site examined.

Conclusion

After IRE-ablation of nerves, the preservation of the architecture of the endoneurium and the proliferation of Schwann cells may enable axonal regeneration as demonstrated after 2 months in this study.

Key Points

Irreversible electroporation (IRE) offers promise for non-thermal tumour ablation.

Preservation of endoneural architecture and proliferation of Schwann cells follow IRE-ablation.

Preservation of architecture and proliferation of Schwann cells may enable axonal regeneration.

Despite morphological regeneration, nerve function remains variable after 2 months.

Keywords

Athermal ablation Irreversible electroporation Sciatic nerve CT-guidance Animal study 

References

  1. 1.
    Lee EW, Chen C, Prieto VE, Dry SM, Loh CT, Kee ST (2010) Advanced hepatic ablation technique for creating complete cell death: irreversible electroporation. Radiology 255:426–433PubMedCrossRefGoogle Scholar
  2. 2.
    Rubinsky B, Onik G, Mikus P (2007) Irreversible electroporation: a new ablation modality–clinical implications. Technol Cancer Res Treat 6:37–48PubMedGoogle Scholar
  3. 3.
    Deodhar A, Monette S, Single WG Jr et al (2011) Renal tissue ablation with irreversible electroporation: preliminary results in a porcine model. Urology 77:754–760PubMedCrossRefGoogle Scholar
  4. 4.
    Schoellnast H, Monette S, Ezell PC et al (2011) Acute and subacute effects of irreversible electroporation on nerves: experimental study in a pig model. Radiology 260:421–427PubMedCrossRefGoogle Scholar
  5. 5.
    Geuna S, Raimondo S, Ronchi G et al (2009) Chapter 3: histology of the peripheral nerve and changes occurring during nerve regeneration. Int Rev Neurobiol 87:27–46Google Scholar
  6. 6.
    Li W, Fan Q, Ji Z, Qiu X, Li Z (2011) The effects of irreversible electroporation (IRE) on nerves. PLoS One 6:e18831PubMedCrossRefGoogle Scholar
  7. 7.
    Arena CB, Sano MB, Rossmeisl JH Jr et al (2011) High-frequency irreversible electroporation (H-FIRE) for non-thermal ablation without muscle contraction. Biomed Eng Online 10:102PubMedCrossRefGoogle Scholar

Copyright information

© European Society of Radiology 2012

Authors and Affiliations

  • Helmut Schoellnast
    • 1
    • 2
  • Sebastien Monette
    • 3
  • Paula C. Ezell
    • 4
  • Majid Maybody
    • 1
  • Joseph P. Erinjeri
    • 1
  • Michael D. Stubblefield
    • 5
  • Gordon Single
    • 6
  • Stephen B. Solomon
    • 1
  1. 1.Department of RadiologyMemorial Sloan-Kettering Cancer CenterNew YorkUSA
  2. 2.Department of RadiologyMedical University of GrazGrazAustria
  3. 3.Laboratory of Comparative PathologyMemorial Sloan-Kettering Cancer Center, Weill Cornell Medical College, and the Rockefeller UniversityNew YorkUSA
  4. 4.Research Animal Resource CenterMemorial Sloan-Kettering Cancer Center and Weill Cornell Medical CollegeNew YorkUSA
  5. 5.Department of Neurology, Rehabilitation Medicine ServiceMemorial Sloan-Kettering Cancer CenterNew YorkUSA
  6. 6.AngioDynamics IncQueensburyUSA

Personalised recommendations