European Radiology

, Volume 15, Issue 11, pp 2268–2275 | Cite as

Magnetic resonance characterization of tumor microvessels in experimental breast tumors using a slow clearance blood pool contrast agent (carboxymethyldextran-A2-Gd-DOTA) with histopathological correlation

  • Anda Preda
  • Viktor Novikov
  • Martina Möglich
  • Eugenia Floyd
  • Karl Turetschek
  • David M. Shames
  • Timothy P. L. Roberts
  • Claire Corot
  • Wayne O. Carter
  • Robert C. BraschEmail author


Carboxymethyldextran (CMD)-A2-Gd-DOTA, a slow clearance blood pool contrast agent with a molecular weight of 52.1 kDa, designed to have intravascular residence for more than 1 h, was evaluated for its potential to characterize and differentiate the microvessels of malignant and benign breast tumors. Precontrast single-slice inversion-recovery snapshot FLASH and dynamic contrast-enhanced MRI using an axial T1-weighted three-dimensional spoiled gradient recalled sequence was performed in 30 Sprague-Dawley rats with chemically induced breast tumors. Endothelial transfer coefficient and fractional plasma volume of the breast tumors were estimated from MRI data acquired with CMD-A2-Gd-DOTA enhancement injected at a dose of 0.1 mmol Gd/kg body weight using a two-compartment bidirectional model of the tumor tissue. The correlation between MRI microvessel characteristics and histopathological tumor grade was determined using the Scarff-Bloom-Richardson method. Using CMD-A2-Gd-DOTA, no significant correlations were found between the MR-estimated endothelial transfer coefficient or plasma volumes with histological tumor grade. Analysis of CMD-A2-Gd-DOTA-enhanced MR kinetic data failed to demonstrate feasibility for the differentiation of benign from malignant tumors or for image-based tumor grading.


Magnetic resonance imaging Contrast media Carboxymethyldextran-A2-Gd-DOTA Breast neoplasms Dynamic contrast enhancement 



This research was supported by funds received from the National Cancer Institute, grant CA82923 and from the Cancer Research Fund, State of California, under interagency agreement 97-12013 (University of California contract 98-00924V). A.P. was supported in part by the Drie Lichten and the Johan Vermeij Foundations, The Netherlands.


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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Anda Preda
    • 1
    • 2
  • Viktor Novikov
    • 1
  • Martina Möglich
    • 1
  • Eugenia Floyd
    • 3
  • Karl Turetschek
    • 1
  • David M. Shames
    • 1
  • Timothy P. L. Roberts
    • 1
  • Claire Corot
    • 4
  • Wayne O. Carter
    • 3
  • Robert C. Brasch
    • 1
    Email author
  1. 1.Department of Radiology, Center for Pharmaceutical and Molecular ImagingUniversity of California San FranciscoSan FranciscoUSA
  2. 2.Department of RadiologyErasmus University Medical CenterRotterdamThe Netherlands
  3. 3.Pfizer Central ResearchGrotonUSA
  4. 4.Guerbet LaboratoriesAulnay sous BoisFrance

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