Genetic structure of Galdieria populations from Iceland

  • Ayla Eren
  • Manuela Iovinella
  • Hwan Su Yoon
  • Paola Cennamo
  • Mario de Stefano
  • Olga de Castro
  • Claudia Ciniglia
Original Paper
  • 29 Downloads

Abstract

Galdieria is a photosynthetic unicellular protist, inhabiting thermoacidic environments around the world. The synchronicity of these thermoacidophilic algae with their extreme habitats makes them unable to thrive in different ecological conditions. The genetic structure of Galdieria populations has not yet been studied. In this report, the level of genetic diversity and structure of five Galdieria populations from Iceland were assessed through both random amplified polymorphic DNA (RAPD) markers, and a partial calmodulin gene fragment (previously used to assess the population structure of these extremophilic algae). The level of population differentiation from both the RAPD and CaM markers was estimated using PLP, percentage of polymorphic loci; H, the expected heterozygosity; I, Shannon’s information index, Ks, Kst. The migration ability of the Galdieria populations was suggested by the high level of genetic variations scored within each Galdieria population and by the small number of polymorphisms detected between the different Icelandic populations. The low genetic diversity between the two species was highlighted by RAPD and calmodulin markers, suggesting for the first time an interspecific genetic flow between species as strategy to evolve in stressful environments.

Keywords

CaM gene Galdieria Genetic structure Microalgae RAPD Iceland Rhodophyta 

Notes

Acknowledgements

We are thankful to Dr Gudmundur Vidir Helgason for hospitality and laboratory support at the Sandgerdi Marine Centre (SMC). The stay at the SMC was supported by the program “Improving Human Potential: Access to Research Infrastructure” provided by the European Community. We are grateful to Prof. Antonino Pollio for the logistical support of the algal cultures which are maintained at the Algal Collection of University of Naples “Federico II”. Many thanks to Dr Rachael Oakenfull for the English editing of the manuscript. We are also grateful to Prof. Hwan Su Yoon for calmodulin gene sequencing, which was supported by the Korean Rural Development Administration Next-generation BioGreen21 (PJ009525), the National Research Foundation of Korea (MEST: 2014R1A2A2A01003588), and US National Science Foundation (DEB 1317114) to HSY.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Sakarya UniversitySakaryaTurkey
  2. 2.Department of BiologyUniversity of YorkYorkUK
  3. 3.Department of Biological SciencesSungkyunkwan UniversitySeoulSouth Korea
  4. 4.Faculty of LettersUniversity of Naples Suor Orsola BenincasaNaplesItaly
  5. 5.Department of Environmental, Biological and Pharmaceutical Science and TechnologyUniversity of Campania “L. Vanvitelli”CasertaItaly
  6. 6.Department of BiologyUniversity of Federico IINaplesItaly

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