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Polar Biology

, Volume 40, Issue 11, pp 2297–2305 | Cite as

Blood O2 affinity of a large polar elasmobranch, the Greenland shark Somniosus microcephalus

  • N. A. Herbert
  • P. V. Skov
  • B. Tirsgaard
  • P. G. Bushnell
  • R. W. Brill
  • C. Harvey Clark
  • J. F. Steffensen
Original Paper

Abstract

The Greenland shark (Somniosus microcephalus. Bloch & Schneider 1801) is a polar elasmobranch that is hypothesised to possess a unique metabolic physiology due to its extreme large size, the cold waters it inhabits and its slow swimming lifestyle. Our results therefore provide the first insight into the metabolic physiology of this unique shark, with a focus on blood O2 affinity. An evaluation of blood O2 affinity at 2 °C using tonometry revealed a P 50 of 11.7 mmHg at a PCO2 of 2.25 mmHg and a Bohr effect (binding sensitivity of blood to pH, ϕ = Δlog P 50/ΔpH) of −0.26. A comparative evaluation of blood O2 affinity across elasmobranch fishes suggests that S. microcephalus has a high blood O2 affinity (i.e., low P 50) and a small Bohr effect but these are common traits in sluggish elasmobranch fishes, with little evidence for any relationship of blood O2 affinity to the low metabolic rates, low environmental temperatures, or large body mass of S. microcephalus. After gathering this physiology data, a subsidiary aim attempted to understand whether a warming scenario would impose a negative effect on blood O2 binding. Incubating blood to a slightly elevated temperature of 7 °C resulted in a small but significant reduction of blood O2 affinity, but no significant change in the Bohr effect. The Hill’s cooperativity coefficient (n H) was also small (1.6–2.2) and unaffected by either PCO2 or temperature. The moderate sensitivity of Greenland shark blood O2 affinity to warming potentially implies little vulnerability of functional O2 supply to the temperature changes associated with the regular vertical movements of this species or warming of polar seas resulting from directional climate change.

Keywords

Metabolism Oxygen Climate change Oxygen transport Haemoglobin Water depth Swimming 

Notes

Acknowledgements

Considerable gratitude and appreciation are extended to the following: the crew of RV Dana for the skilled logistical support and cooperation provided across the whole 10-day research period. Financial support from the Danish Center for Marine Research, the Carlsberg Foundation, Save Our Seas Foundation (Grant No. P219), and KVUG (Commission for Scientific Research in Greenland). Mention of trade names or commercial companies is for identification purposes only and does not imply endorsement by the National Marine Fisheries Service, NOAA. The views expressed are those of the authors and do not necessarily reflect the views of NOAA or any of its sub-agencies.

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • N. A. Herbert
    • 1
  • P. V. Skov
    • 2
  • B. Tirsgaard
    • 3
  • P. G. Bushnell
    • 4
  • R. W. Brill
    • 5
  • C. Harvey Clark
    • 6
  • J. F. Steffensen
    • 3
  1. 1.Leigh Marine Laboratory, Institute of Marine ScienceThe University of AucklandWarkworthNew Zealand
  2. 2.National Institute of Aquatic ResourcesTechnical University of DenmarkHirtshalsDenmark
  3. 3.Marine Biological SectionUniversity of CopenhagenHelsingørDenmark
  4. 4.Department of Biological SciencesIndiana University South BendSouth BendUSA
  5. 5.James J. Howard Marine Sciences Laboratory, Northeast Fisheries Science Center, National Marine Fisheries ServiceNOAAHighlandsUSA
  6. 6.Department VeterinarianDalhousie UniversityHalifaxCanada

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