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Polar Biology

, Volume 35, Issue 9, pp 1439–1445 | Cite as

Occurrence and diversity of fungal entomopathogens in soils of low and high Arctic Greenland

  • Nicolai V. Meyling
  • Niels M. Schmidt
  • Jørgen Eilenberg
Short Note

Abstract

Knowledge of the occurrence, distribution and diversity of pathogens of insects and arachnids (entomopathogens) in the Arctic is very limited. Climate change is expected to affect Arctic terrestrial arthropod communities and therefore also host–pathogen interactions, given that entomopathogens are present. We conducted a survey of fungal entomopathogens in soil samples collected at four localities in Greenland; two at low Arctic sites (Ritenbenk and Disko Island) and two at sites in the high Arctic (Zackenberg and Danmarkshavn). Fungi were isolated from soil samples using larvae of the insect species Galleria mellonella (Lepidoptera) and Tenebrio molitor (Coleoptera) as baits providing evidence that the fungal isolates were indeed entomopathogenic. Five fungal species (Ascomycota; Hypocreales) were found: Isaria fumosorosea Wize, Isaria farinosa (Holmsk.) Fr., Beauveria bassiana (Bals.) Vuill., Beauveria pseudobassiana Rehner and Humber and Tolypocladium inflatum W. Gams (syn. = T. niveum). I. farinosa was found at all four localities, while I. fumosorosea was detected in single samples at each of three localities including both high Arctic sites. Only the locality on Disko Island revealed B. bassiana, whereas B. pseudobassiana was isolated at the three other sites. T. inflatum was only found on Disko Island and only isolated with T. molitor as a bait insect. The results document that fungal entomopathogens are widely distributed in the soil environment in Greenland. Entomopathogens should therefore be included in future studies of arthropod ecology in the Arctic.

Keywords

Insect bait method Beauveria pseudobassiana Isaria farinosa Isaria fumosorosea Tolypocladium inflatum 

Notes

Acknowledgments

We thank Nette Levermann, Katrine Raundrup, Anders Mosbech and Holger Philipsen for assistance with the collection of soil samples and Anja A. Wynns for assistance with obtaining digital micrographs. We are grateful to Dr. David Nash for correcting the English and preparing map. Karen Svendsen and Christina Wolsted provided technical assistance.

References

  1. Bergero R, Girlanda M, Varese GC, Intili D, Luppi AM (1999) Psychrooligotrophic fungi from Arctic soils of Franz Joseph Land. Polar Biol 21:361–368CrossRefGoogle Scholar
  2. Bissett J, Parkinson D (1979) The distribution of fungi in some alpine soils. Can J Bot 57:1609–1629CrossRefGoogle Scholar
  3. Christensen T, Payne JF, Schmidt NM, Madsen J, Taylor JJ, Doyle M, Gill M, Nymand J, Svoboda M, Rosa C, Shuchman B, Soloviev M, Aronsson M, Paakko E, Fosaa AM, Heidmarsson S, Solberg BØ (2011) Terrestrial Expert Monitoring Plan—background paper. A Supporting Publication to the CBMP Framework Document. CAFF International Secretariat, CAFF Monitoring Series Report No. 6. ISBN 978-9935-431-11-0. 74 pGoogle Scholar
  4. Danks HV (2004) Seasonal adaptations in Arctic insects. Integr Comp Biol 44:85–94PubMedCrossRefGoogle Scholar
  5. Doberski JW (1981) Comparative laboratory studies on three fungal pathogens of the elm bark beetle Scolytus scolytus: effects of temperature and humidity on infection by Beauveria bassiana, Metarhizium anisopliae, and Paecilomyces farinosus. J Invertebr Pathol 37:195–200CrossRefGoogle Scholar
  6. Flanagan PW, Scarborough AM (1974) Physiological groups of decomposer fungi on tundra plant remains. In: Holding AJ, Heal OW, Maclean SF, Flanagan PW (eds) Soil organisms and decomposition in Tundra. Tundra Biome Steering Commitee, Stockholm, pp 159–181Google Scholar
  7. Hajek AE, St. Leger RJ (1994) Interactions between fungal pathogens and insect hosts. Annu Rev Entomol 39:293–322CrossRefGoogle Scholar
  8. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
  9. Hesketh H, Roy HE, Eilenberg J, Pell JK, Hails RS (2010) Challenges in modelling complexity of fungal entomopathogens in semi-natural populations of insects. Biocontrol 55:55–73CrossRefGoogle Scholar
  10. Hodge K, Krasnoff SB, Humber RA (1996) Tolypocladium inflatum is the anamorph of Cordyceps subsessilis. Mycologia 88:715–719CrossRefGoogle Scholar
  11. Høye TT, Forchhammer MC (2008) Phenology of high-arctic arthropods: effects of climate on spatial, seasonal, and inter-annual variation. Adv Ecol Res 40:299–324CrossRefGoogle Scholar
  12. Humber RA (1997) Fungi: Identification. In: Lacey LA (ed) Manual of techniques in insect pathology. Academic Press, San Diego, pp 153–185Google Scholar
  13. Keller S, Zimmerman G (1989) Mycopathogens of soil insects. In: Wilding N, Collins NM, Hammond PM, Webber JF (eds) Insect-fungus interactions. Academic Press, London, pp 240–270Google Scholar
  14. Klingen I, Eilenberg J, Meadow R (2002) Effects of farming system, field margins and bait insect on the occurrence of insect pathogenic fungi in soils. Agric Ecosyst Environ 91:191–198CrossRefGoogle Scholar
  15. Luangsa-Ard JJ, Hywel-Jones NL, Manoch L, Samson RA (2005) On the relationships of Paecilomyces sect. Isarioidea species. Mycol Res 109:581–589PubMedCrossRefGoogle Scholar
  16. Mietkiewski R, Tkaczuk C (1998) The spectrum and frequency of entomopathogenic fungi in litter, forest soil and arable soil. IOBC Bull 21(4):41–44Google Scholar
  17. Meyling NV, Pilz C, Keller S, Widmer F, Enkerli J (2012) Diversity of Beauveria spp. isolates from pollen beetles Meligethes aeneus in Switzerland. J Invertebr Pathol 109:76–82PubMedCrossRefGoogle Scholar
  18. Meyling NV, Eilenberg J (2006) Occurrence and distribution of soil borne entomopathogenic fungi within a single organic agroecosystem. Agric Ecosyst Environ 113:336–341CrossRefGoogle Scholar
  19. Meyling NV, Eilenberg J (2007) Ecology of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae in temperate agroecosystems: potential for conservation biological control. Biol Control 43:145–155CrossRefGoogle Scholar
  20. Nikoh N, Fukatsu T (2001) Evolutionary dynamics of multiple group I introns in nuclear ribosomal RNA genes of endoparasitic fungi of the genus Cordyceps. Mol Biol Evol 18:1631–1642PubMedCrossRefGoogle Scholar
  21. Oddsdottir ES, Nielsen C, Sen R, Harding S, Eilenberg J, Halldorsson G (2010) Distribution patterns of soil entomopathogenic and birch symbiotic ectomycorrhizal fungi across native woodland and degraded habitats in Iceland. Icel Agric Sci 23:37–49Google Scholar
  22. Rehner SA, Minnis AM, Sung G-H, Luangsa-ard JJ, Devotto L, Humber RA (2011) Phylogeny and systematics of the anamorphic, entomopathogenic genus Beauveria. Mycologia 103:1055–1073PubMedCrossRefGoogle Scholar
  23. Robinson CH (2001) Cold adaptation in Arctic and Antarctic fungi. New Phytol 151:341–353CrossRefGoogle Scholar
  24. Stenrud Ø, Hywel-Jones NL, Schumacher T (2005) Towards a phylogenetic classification of Cordyceps: ITS nrDNA sequence data confirm divergent lineages and paraphyly. Mycol Res 109:41–56CrossRefGoogle Scholar
  25. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA 4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599PubMedCrossRefGoogle Scholar
  26. Tulip I, Schekkerman H (2008) Has prey availability for Arctic birds advanced with climate change? Hindcasting the abundance of tundra arthropods using weather and seasonal variation. Arctic 61:48–60Google Scholar
  27. Vänninen I (1995) Distribution and occurrence of four entomopathogenic fungi in Finland: effect of geographical location, habitat type and soil type. Mycol Res 100:93–101CrossRefGoogle Scholar
  28. Vega FE, Meyling NV, Luangsa-ard JJ, Blackwell M (2012) Fungal entomopathogens. In: Vega FE, Kaya H (eds) Insect pathology. Academic Press, Elsevier Inc., San Diego, pp 171–220Google Scholar
  29. Vernon P, Vannier G, Trehen P (1998) A comparative approach to the entomological diversity of polar regions. Acta Oecol 19:303–308CrossRefGoogle Scholar
  30. White TJ, Bruns TD, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols. Academic Press, San Diego, pp 315–322Google Scholar
  31. Widden P, Parkinson D (1979) Populations of fungi in a high arctic ecosystem. Can J Bot 57:2408–2417CrossRefGoogle Scholar
  32. Yokoyama E, Arakawa M, Yamagishi K, Hara A (2006) Phylogenetic and structural analyses of the mating-type loci in Clavicipitaceae. FEMS Microbiol Lett 264:182–191PubMedCrossRefGoogle Scholar
  33. Zimmermann G (1986) The Galleria bait method for detection of entomopathogenic fungi in soil. J Appl Entomol 102:213–215CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Nicolai V. Meyling
    • 1
  • Niels M. Schmidt
    • 2
  • Jørgen Eilenberg
    • 1
  1. 1.Department of Agriculture and EcologyUniversity of CopenhagenFrederiksberg CDenmark
  2. 2.Department of BioscienceAarhus UniversityRoskildeDenmark

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