Polar Biology

, Volume 35, Issue 9, pp 1297–1310 | Cite as

DNA barcodes and species identifications in Ross Sea and Southern Ocean fishes

  • P. J. Smith
  • D. Steinke
  • A. Dettai
  • P. McMillan
  • D. Welsford
  • A. Stewart
  • R. D. Ward
Original Paper

Abstract

The Southern Ocean occupies about 10 % of the world’s oceans but has low species richness with only ~1.5 % of the marine fishes. Within the Southern Ocean, the Ross Sea region is one of the least exploited sea areas in the world, but is subject to commercial fishing. The fauna are not well known, and preliminary IPY molecular studies have indicated that species diversity has been underestimated in this region. DNA barcodes of fishes from the Ross Sea region were compared with barcodes of fishes from the Atlantic and Indian Ocean sectors of the Southern Ocean. Barcoding resolved 87.5 % of 112 species that typically exhibited high inter-specific divergences. Intra-specific divergence was usually low with shared haplotypes among regions. The Zoarcid Ophthalmolycus amberensis showed shallow divergences (0.1 %) within the Ross Sea and Australian Antarctic Territory but high inter-region divergence (2 %), indicative of cryptic species. Other potential cryptic species with high intra-specific divergences were found in Notolepis coatsi and Gymnoscopelus bolini. In contrast, several taxa showed low inter-specific divergences and shared haplotypes among morphological species. COI provided limited phylogenetic resolution of the genera Pogonophryne and Bathydraco. Trematomus loennbergii and T. lepidorhinus shared COI haplotypes, as previously noted in other regions, as did Cryodraco antarcticus and C. atkinsoni. There was a marked lack of congruence between morphological descriptions and COI divergences among the Ross Sea liparids with shallow or zero divergences among recently described species. Barcodes for the Ross Sea fishes highlighted several initial misidentifications that were corrected when specimens were re-examined.

Keywords

DNA barcode Southern Ocean Fishes Species identification 

Supplementary material

300_2012_1173_MOESM1_ESM.xls (240 kb)
Appendix 1 Southern Ocean fishes barcoded listed by Species, Specimen Reference Number, GenBank Accession Number, Bold Process Number, and Institute storing specimens. The list excludes published data on Macrourus, available in Smith et al. (2011) (XLS 240 kb)
300_2012_1173_MOESM2_ESM.pdf (38 kb)
Appendix 2 COI relationships among the Artedidraconidae (154 specimens, including those identified to genus level only) from the Southern Ocean; unrooted ML tree. The scale bar represents an interval of the K2P model; numbers at nodes are bootstrap percentages (>75%). Sequence numbers represent BOLD Process Number, GenBank Accession Number, followed by Registration Number, and species name (PDF 37 kb)
300_2012_1173_MOESM3_ESM.pdf (43 kb)
Appendix 3 COI relationships among Trematomus specimens (181 specimens, including those identified to genus level only); unrooted ML tree. The scale bar represents an interval of the K2P model; numbers at nodes are bootstrap percentages (>75%). Sequence numbers represent BOLD Process Number, GenBank Accession Number, followed by Registration Number, and species name (PDF 42 kb)
300_2012_1173_MOESM4_ESM.pdf (39 kb)
Appendix 4 COI relationships among the Bathydraconidae (168 specimens, including those identified to genus level only) from the Southern Ocean; unrooted ML tree. The scale bar represents an interval of the K2P +G model; numbers at nodes are bootstrap percentages (>75%). Sequence numbers represent BOLD Process Number, GenBank Accession Number, followed by Registration Number, and species name (PDF 39 kb)

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • P. J. Smith
    • 1
  • D. Steinke
    • 2
  • A. Dettai
    • 3
  • P. McMillan
    • 4
  • D. Welsford
    • 5
  • A. Stewart
    • 6
  • R. D. Ward
    • 7
  1. 1.National Museum VictoriaMelbourneAustralia
  2. 2.Canadian Centre for DNA Barcoding, Biodiversity Institute of OntarioUniversity of GuelphGuelphCanada
  3. 3.Muséum national d‘Histoire naturelle, Département Systématique et EvolutionUMR 7138Paris, Cedex 05France
  4. 4.National Institute of Water and Atmospheric Research Ltd.WellingtonNew Zealand
  5. 5.Australian Antarctic DivisionKingstonAustralia
  6. 6.Museum of New Zealand Te Papa TongarewaWellingtonNew Zealand
  7. 7.Wealth from Oceans FlagshipCSIRO Marine and Atmospheric ResearchHobartAustralia

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