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Polar Biology

, 34:1657 | Cite as

Hypolithic microbial communities of quartz rocks from Miers Valley, McMurdo Dry Valleys, Antarctica

  • Nuraan Khan
  • Marla Tuffin
  • William Stafford
  • Craig Cary
  • Donnabella C. Lacap
  • Stephen B. Pointing
  • Don CowanEmail author
Original Paper

Abstract

The McMurdo Dry Valleys region of eastern Antarctica is a cold desert that presents extreme challenges to life. Hypolithic microbial colonisation of the subsoil surfaces of translucent quartz rocks represent a significant source of terrestrial biomass and productivity in this region. Previous studies have described hypoliths as dominated by cyanobacteria. However, hypoliths that occur in the lower Dry Valleys such as the Miers, Garwood and Marshall Valleys are unusual as they are not necessarily cyanobacteria-dominated. These hypoliths support significant eukaryal colonisation by fungi and mosses in addition to cyanobacteria-dominated bacterial assemblages and so have considerable ecological value in this barren landscape. Here, we characterise these novel hypoliths by analysis of environmental rRNA gene sequences. The hypolithic community was demonstrated to be distinct from the surrounding soil and non-translucent rocks. Hypoliths supported cyanobacterial signatures from the Oscillatoriales and Nostocales. Other heterotrophic bacterial signatures were also recovered, and these were phylogenetically diverse and spanned 8 other bacterial phyla. Archaeal phylotypes recovered were phylogenetically affiliated with the large group of unclassified, uncultured Crenarcheota. Eukaryal phylotypes indicated that free-living ascomycetous fungi, chlorophytes and mosses (Bryum sp.) were all supported by these hypoliths, and these are thought to be responsible for the extensive eukaryotic biomass that develops around quartz rocks.

Keywords

Hypolith Antarctic Dry Valleys Microbial colonisation DGGE Phylogenetics 

Notes

Acknowledgments

This research was undertaken under the auspices of the South African national Antarctic Programme; the New Zealand Antarctic Research Programme under Antarctica New Zealand and the Waikato University Antarctic Terrestrial Biology Research Programme. The authors gratefully acknowledge funding from the National Research Foundation (SA), the Hong Kong Research Grants Council (7733/08) and the NZ Ministry of Science and Innovation (Terrestrial Antarctic Biocomplexity Survey, nzTABS). The authors declare that they have no conflict of interest.

Supplementary material

300_2011_1061_MOESM1_ESM.doc (3.8 mb)
Supplementary material 1 (DOC 3884 kb)

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Nuraan Khan
    • 1
    • 2
  • Marla Tuffin
    • 1
  • William Stafford
    • 1
    • 4
  • Craig Cary
    • 3
  • Donnabella C. Lacap
    • 5
  • Stephen B. Pointing
    • 5
  • Don Cowan
    • 1
    Email author
  1. 1.Department of Biotechnology, Institute for Microbial Biotechnology and MetagenomicsUniversity of the Western CapeBellvilleSouth Africa
  2. 2.Biocatalysis and Technical Biology Research GroupCape Peninsula University of TechnologyBellvilleSouth Africa
  3. 3.Thermophile Research Unit, Department of Biological SciencesUniversity of WaikatoHamiltonNew Zealand
  4. 4.Natural Resources and EnvironmentCSIRStellenboschSouth Africa
  5. 5.The University of Hong KongHong Kong SARChina

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