Polar Biology

, Volume 34, Issue 6, pp 907–919 | Cite as

Phylogenetic diversity of sediment bacteria in the northern Bering Sea

Original Paper
First Online:
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Abstract

The bacterial diversity in sediments from the northern Bering Sea was investigated by culture-independent approaches. Community fingerprint analysis by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) revealed that sediment at two deep stations (DBSE and DBS1, >400 m in depth) harbored a bacterial community distinct from the sediments collected at shallow stations (<150 m in depth) on the continental shelf. Three 16S rRNA gene clone libraries for sediments collected from shallow to deep water stations (NEC5, DBSE and DBS1, respectively) were established. Sediment collected at the deepest station DBS1 showed the highest diversity index value. Sequences fell into 19 major lineages of the domain Bacteria: Alpha-, Beta-, Gamma-, Delta- and Epsilonproteobacteria, Bacteroidetes, Acidobacteria, Actinobacteria, Firmicutes, Planctomycetes, Nitrospirae, Verrucomicrobia, Chloroflexi, Chlorobi, Spirochaetes, Cyanobacteria (or chloroplasts), and candidate divisions OP8, TM6, and WS3. A small fraction of retrieved sequences (1.8%) did not fall into any taxonomic division. Deltaproteobacteria (30%) was the dominant phylum in the three libraries, followed by Gammaproteobacteria (21%) and Acidobacteria (16%). The percentages of cloned sequences with the highest similarity to reported sequences below 97 and 93% were 48.1 and 24.3%, respectively. A large number of phylotypes affiliated with bacteria that play important roles in the carbon, sulfur, and nitrogen cycles suggest an important link of bacteria to the matter cycling in these subarctic sediments.

Keywords

Bacterial diversity PCR-DGGE 16S rRNA gene clone library Sediment Northern Bering Sea 
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Notes

Acknowledgments

We appreciate the assistance of the US Coast Guard crew and the scientists who collected samples for us on the Healy0702 cruise. We are also grateful to anonymous reviewers for comments and suggested modifications to the text. This work was supported by the National Natural Science Foundation of China (grant no. 40676002, 40930847, 41076131 and 30940002), the US National Science Foundation (grant no. NSF-OPP-ARC-0454454 to JM Grebmeier and LW Cooper), the Emphasis Project Fund of National Scientific and Technical Support Plan (grant no. 2006BAB18B07), the International Science and Technology Cooperation Program (grant no. 2008DFA20420), and China’s Action Plan for the International Polar Year (IPY) supported by the Chinese Arctic and Antarctic Administration (CAA).

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Yinxin Zeng
    • 1
    • 2
  • Yang Zou
    • 2
  • Bo Chen
    • 1
  • Jacqueline M. Grebmeier
    • 3
  • Huirong Li
    • 1
  • Yong Yu
    • 1
  • Tianling Zheng
    • 2
  1. 1.Key Laboratory for Polar Science of State Oceanic AdministrationPolar Research Institute of ChinaShanghaiChina
  2. 2.Key Laboratory of MOE for Coast and Wetland Ecosystem, School of Life SciencesXiamen UniversityXiamenChina
  3. 3.Chesapeake Biological LaboratoryUniversity of Maryland Center for Environmental ScienceSolomonsUSA

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