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Polar Biology

, Volume 34, Issue 6, pp 933–937 | Cite as

Contaminant loading in remote Arctic lakes affects cellular stress-related proteins expression in feral charr

  • Steve Wiseman
  • Even H. Jørgensen
  • Alec G. Maule
  • Mathilakath M. VijayanEmail author
Short Note

Abstract

The remote Arctic lakes on Bjørnøya Island, Norway, offer a unique opportunity to study possible affect of lifelong contaminant exposure in wild populations of landlocked Arctic charr (Salvelinus alpinus). This is because Lake Ellasjøen has persistent organic pollutant (POP) levels that are significantly greater than in the nearby Lake Øyangen. We examined whether this differential contaminant loading was reflected in the expression of protein markers of exposure and effect in the native fish. We assessed the expressions of cellular stress markers, including cytochrome P4501A (Cyp1A), heat shock protein 70 (hsp70), and glucocorticoid receptor (GR) in feral charr from the two lakes. The average polychlorinated biphenyl (PCB) load in the charr liver from Ellasjøen was approximately 25-fold higher than in individuals from Øyangen. Liver Cyp1A protein expression was significantly higher in individuals from Ellasjøen compared with Øyangen, confirming differential PCB exposure. There was no significant difference in hsp70 protein expression in charr liver between the two lakes. However, brain hsp70 protein expression was significantly elevated in charr from Ellasjøen compared with Øyangen. Also, liver GR protein expression was significantly higher in the Ellasjøen charr compared with Øyangen charr. Taken together, our results suggest changes to cellular stress-related protein expression as a possible adaptation to chronic-contaminant exposure in feral charr in the Norwegian high-Arctic.

Keywords

Cyp1A hsp70 Glucocorticoid receptor Salvelinus alpinus PCB Salmonid Fish 

Notes

Acknowledgments

We thank Guttorm Christensen and Anita Evenset who collected the fish tissue samples for us at Bjørnøya Island. The study was funded by the Norwegian Research Council, project no. 114263/720 and the Natural Sciences and Engineering Research Council (NSERC, Canada) discovery grant.

References

  1. Brevik EM (1978) Gas chromatographic method for the determination of organochlorine pesticides in human milk. Bull Environ Contam Toxicol 19:281–286PubMedCrossRefGoogle Scholar
  2. EPA (1996) Test methods for evaluating solid waste (SW-846), methods 8081A and 8082. U.S. EPA, BostonGoogle Scholar
  3. Evenset A, Christensen GN, Skotvold T, Fjeld E, Schlabach M, Wartena E, Gregor D (2004) A comparison of organic contaminants in two high Arctic lake ecosystems, Bjørnøya (Bear Island), Norway. Sci Total Environ 318:125–141PubMedCrossRefGoogle Scholar
  4. Evenset A, Christensen GN, Kallenborn R (2005) Selected chlorobornanes, polychlorinated naphthalenes and brominated flame retardants in Bjørnøya (Bear Island) freshwater biota. Environ Pollut 136:419–430PubMedCrossRefGoogle Scholar
  5. Evenset A, Christensen GN, Carroll J, Zaborska A, Berger U, Herzke D, Gregor D (2007) Historical trends in persistent organic pollutants and metals recorded in sediment from Lake Ellasjøen, Bjørnøya, Norwegian Arctic. Environ Pollut 146:196–205PubMedCrossRefGoogle Scholar
  6. Hahn ME (2002) Biomarkers and bioassays for detecting dioxin-like compounds in the marine environment. Environ Toxicol Chem 22:49–69Google Scholar
  7. Hightower LE (1991) Heat shock, stress proteins, chaperones, and proteotoxicity. Cell 66:191–197PubMedCrossRefGoogle Scholar
  8. Hontela A (2005) Adrenal toxicology: environmental pollutants and the HPI axis. In: Moon TW, Mommsen TP (eds) Biochemical and molecular biology of fishes, vol 6 environmental toxicology. Elsevier, Amsterdam, pp 331–363Google Scholar
  9. Iwama GK, Thomas PT, Forsyth RB, Vijayan MM (1998) Heat shock protein expression in fish. Rev Fish Biol Fish 8:35–56CrossRefGoogle Scholar
  10. Janz DM, McMaster ME, Munkittrick KR, Van Der Kraak G (1997) Elevated ovarian follicular apoptosis and heat shock protein-70 expression in white sucker exposed to bleached kraft pulp mill effluent. Toxicol Appl Pharmacol 147:391–398PubMedCrossRefGoogle Scholar
  11. Jørgensen EH, Vijayan MM, Killie JE, Aluru N, Aas-Hansen O, Maule A (2006) Toxicokinetics and effects of PCBs in Arctic fish: a review of studies on Arctic charr. J Toxicol Environ Health A 69:37–52PubMedCrossRefGoogle Scholar
  12. Klemetsen A, Grotnes PE, Holthe H, Kristoffersen K (1985) Bear Island charr. Nordic J Freshw Res 62:98–119Google Scholar
  13. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685PubMedCrossRefGoogle Scholar
  14. Mommsen TP, Vijayan MM, Moon TW (1999) Cortisol in teleosts: dynamics, mechanisms of action, and metabolic regulation. Rev Fish Biol Fish 9:211–268CrossRefGoogle Scholar
  15. Parsell DA, Lindquist S (1993) The function of heat-shock proteins in stress tolerance: degradation and reactivation of damaged proteins. Annu Rev Genet 27:437–496PubMedCrossRefGoogle Scholar
  16. Sathiyaa R, Vijayan MM (2003) Autoregulation of glucocorticoid receptor by cortisol in rainbow trout hepatocytes. Am J Physiol Cell Physiol 284:1508–1515Google Scholar
  17. Skotvold T, Wartena EMM, Schlaback M (1998) Persistent organic contaminants in Arctic charr (Salvelinus alpinus) on Bear Island. Organohal Compd 39:411–414Google Scholar
  18. Vijayan MM, Prunet P, Boone AN (2005) Xenobiotic impact on corticosteroid signaling. In: Moon TW, Mommsen TP (eds) Biochemical and molecular biology of fishes, vol 6 environmental toxicology. Elsevier, Amsterdam, pp 365–394Google Scholar
  19. Whitlock JP Jr (1999) Induction of cytochrome P4501A1. Annu Rev Pharmacol Toxicol 39:103–125PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Steve Wiseman
    • 1
  • Even H. Jørgensen
    • 2
  • Alec G. Maule
    • 3
  • Mathilakath M. Vijayan
    • 1
    Email author
  1. 1.Department of BiologyUniversity of WaterlooWaterlooCanada
  2. 2.Norwegian College of Fishery ScienceUniversity of TromsøTromsøNorway
  3. 3.USGSCookUSA

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