Antifungal activity of Arctic and Antarctic bacteria isolates
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Various psychrotolerant bacterial strains with broad-spectrum antimicrobial potential were isolated from a number of Antarctic and Arctic samples (lake sediments, water, as well as faeces, feathers and soils collected in penguin rookeries). Seven isolates from all types of samples exhibited clear antifungal activities against the multidrug-resistant pathogenic yeast strain Candida albicans NCIM 3471. One isolate from the penguin rookery, identified by means of 16S rDNA sequencing as a strain of Enterococcus faecium, showed very strong antimycotic activity against a total of six C. albicans strains. The antibiotic activity was found after ammonium sulphate precipitation and dialysis but was sensitive to proteolytic enzymes.
Keywords16S rDNA Antimicrobial activity Antarctica Candidiasis Inhibition zone
This work was financially supported by the Council of Scientific and Industrial Research (CSIR), New Delhi, India. The samples were provided by the Polar Biology Laboratory NCAOR, Goa, India. Thanks are also due to the Sanmar Specialties for ribotyping. We are thankful to the directors of the Birla Institute of Technology & Science, Pilani KK Birla Goa Campus and National Centre for Antarctic & Ocean Research Goa for providing research facilities. We are particularly grateful to Dr. T. Hoshino (Sapporo) and two anonymous reviewers for their valuable advice that helped a lot in improving the manuscript.
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