Polar Biology

, Volume 32, Issue 1, pp 93–103 | Cite as

Bacterial, archaeal and eukaryotic diversity in Arctic sediment as revealed by 16S rRNA and 18S rRNA gene clone libraries analysis

  • Fei Tian
  • Yong Yu
  • Bo Chen
  • Huirong Li
  • Yu-Feng Yao
  • Xiao-Kui Guo
Original Paper

Abstract

We studied the microbial diversity in the sediment from the Kongsfjorden, Svalbard, Arctic, in the summer of 2005 based on the analysis of 16S rRNA and 18S rRNA gene clone libraries. The sequences of the cloned 16S rRNA and 18S rRNA gene inserts were used to determine the species identity or closest relatives by comparison with sequences of known species. Compared to the other samples acquired in Arctic and Antarctic, which are different from that of ours, the microbial diversity in our sediment is much higher. The bacterial sequences were grouped into 11 major lineages of the domain Bacteria: Proteobacteria (include α-, β-, γ-, δ-, and ε-Proteobacteria); Bacteroidetes; Fusobacteria; Firmicutes; Chloroflexi; Chlamydiae; Acidobacteria; Actinobacteria; Planctomycetes; Verrucomicrobiae and Lentisphaerae. Crenarchaeota were dominant in the archaeal clones containing inserts. In addition, six groups from eukaryotes including Cercozoa, Fungi, Telonema, Stramenopiles, Alveolata, and Metazoa were identified. Remarkably, the novel group Lentisphaerae was reported in Arctic sediment at the first time. Our study suggested that Arctic sediment as a unique habitat may contain substantial microbial diversity and novel species will be discovered.

Keywords

Arctic sediment Microbial diversity 16S rRNA gene 18S rRNA gene 

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Fei Tian
    • 1
  • Yong Yu
    • 2
  • Bo Chen
    • 2
  • Huirong Li
    • 2
  • Yu-Feng Yao
    • 1
  • Xiao-Kui Guo
    • 1
  1. 1.Department of Medical Microbiology and Parasitology, Institutes of Medical SciencesShanghai Jiao Tong University School of MedicineShanghaiChina
  2. 2.State Oceanic Administration Key Laboratory for Polar SciencePolar Research Institute of ChinaShanghaiChina

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