Polar Biology

, Volume 31, Issue 9, pp 1067–1080 | Cite as

Iron, silicate, and light co-limitation of three Southern Ocean diatom species

Original Paper

Abstract

The effect of combined iron, silicate, and light co-limitation was investigated in the three diatom species Actinocyclus sp. Ehrenberg, Chaetoceros dichaeta Ehrenberg, and Chaetoceros debilis Cleve, isolated from the Southern Ocean (SO). Growth of all species was co-limited by iron and silicate, reflected in a significant increase in the number of cell divisions compared to the control. Lowest relative Si uptake and drastic frustule malformation was found under iron and silicate co-limitation in C. dichaeta, while Si limitation in general caused cell elongation in both Chaetoceros species. Higher light intensities similar to SO surface conditions showed a negative impact on growth of C. dichaeta and Actinocyclus sp. and no effect on C. debilis. This is in contrast to the assumed light limitation of SO diatoms due to deep wind driven mixing. Our results suggest that growth and species composition of Southern Ocean diatoms is influenced by a sensitive interaction of the abiotic factors, iron, silicate, and light.

Keywords

Phytoplankton Iron Limitation Diatom Growth High Silicate Polar Frontal Zone 

Notes

Acknowledgments

We thank Jesco Peschutter and Wiebke Schmidt for their help in cell counting, as well as Eike Breitbarth and Peter Croot for helpful comments and discussions. We also would like to thank the two anonymous reviewers for their constructive critics, which have remarkably improved the clarity of the manuscript. This research was funded by the German Research Foundation (DFG) grant PE_565_5.

References

  1. Abelmann A, Gersonde R (1991) Biosiliceous particle flux in the Southern Ocean. Mar Chem 35:503–536CrossRefGoogle Scholar
  2. Admiraal W (1977) Influence of light and temperature on the growth rate of estuarine benthic diatoms in culture. Mar Biol 39:1–9CrossRefGoogle Scholar
  3. Anderson M et al (2004) Light climate and primary productivity in the Arctic, UNIS Publication Series, AB323 Report ISBN 82-481-0010-3, pp 1–95Google Scholar
  4. Banse K (1991) Rates of phytoplankton cell division in the field and in iron enrichment experiments. Limnol Oceanogr 36:1886–1898Google Scholar
  5. Blain S et al (2002) Quantification of algal iron requirements in the subAntarctic Southern Ocean (Indian sector). Deep Sea Res Part II 49:3255–3273CrossRefGoogle Scholar
  6. Brzezinski MA (1992) Cell-cycle effects on the kinetics of silicic acid uptake and resource competition among diatoms. J Plankton Res 14:1511–1539CrossRefGoogle Scholar
  7. Brzezinski MA et al (1990) Silicon availability and cell-cycle progression in marine diatoms. Mar Ecol Prog Ser 67:83–96CrossRefGoogle Scholar
  8. Brzezinski MA et al (2005) Control of silica production by iron and silicic acid during the Southern Ocean Iron Experiment (SOFeX). Limnol Oceanogr 50:810–824Google Scholar
  9. Chisholm SW (1992) Phytoplankton size. In: Falkowski PG, Woodhead AD (eds) Primary productivity and biogeochemical cycles in the sea. Plenum Press, New YorkGoogle Scholar
  10. Claquin P et al (2002) Uncoupling of silicon compared with carbon and nitrogen metabolisms and the role of the cell cycle in continuous cultures of Thalassiosira pseudonana (Bacillariophyceae) under light, nitrogen, and phosphorus control. J Phycol 38:922–930CrossRefGoogle Scholar
  11. Coale KH et al (2003) Phytoplankton growth and biological response to iron and zinc addition in the Ross Sea and Antarctic circumpolar current along 170°W. Deep-Sea Res Part II 50:635–653CrossRefGoogle Scholar
  12. Coale KH et al (2004) Southern Ocean iron enrichment experiment: carbon cycling in high- and low-Si waters. Science 304:408–414PubMedCrossRefGoogle Scholar
  13. Dafner EV, Mordasova NV (1994) Influence of biotic factors on the hydrochemical structure of surface water in the Polar Frontal Zone of the Atlantic Antarctic. Mar Chem 45:137–148CrossRefGoogle Scholar
  14. de Baar HJW et al (1990) On iron limitation of the Southern Ocean: experimental observations in the Weddell and Scotia Seas. Mar Ecol Prog Ser 65:105–122CrossRefGoogle Scholar
  15. de Baar HJW et al (2005) Synthesis of iron fertilisation experiments: from the iron age in the age of enlightenment. J Geophys Res, 110, C09S16. doi: 10.1029/2004JC002601
  16. de La Rocha CL et al (2000) Effects of iron and zinc deficiency on elemental composition and silica production by diatoms, Mar Ecol Prog Ser 195:71–79CrossRefGoogle Scholar
  17. Franck VM et al (2000) Iron and silicic acid concentrations regulate Si uptake north and south of the Polar Frontal Zone in the Pacific Sector of the Southern Ocean. Deep Sea Res Part II 47:3315–3338CrossRefGoogle Scholar
  18. Franck VM et al (2003) Iron and zinc effects on silicic acid and nitrate uptake kinetics in three high-nutrient, low-chlorophyll (HNLC) regions. Mar Ecol Prog Ser 252:15–33CrossRefGoogle Scholar
  19. Grasshoff K et al (1999) Methods of seawater analysis. 3rd edn, Wiley-VCH, WeinheimGoogle Scholar
  20. Greene RM et al (1992) Iron-induced changes in light harvesting and photochemical energy conversion processes in eukaryotic marine algae. Plant Physiol 100:565–575PubMedGoogle Scholar
  21. Greene RM et al (1994) Physiological limitation of phytoplankton photosynthesis in the eastern equatorial Pacific determined from variability in the quantum yield of fluorescence. Limnol Oceanogr 39:1061–1074CrossRefGoogle Scholar
  22. Harrison PJ et al (1977) Marine diatoms grown in chemostats under silicate or ammonium limitation. III. Cellular chemical composition and morphology of Chaetoceros debilis, Skeletonema costatum, and Thalassosira gravida. Mar Biol 43:19–31CrossRefGoogle Scholar
  23. Hillebrand H et al (1999) Biovolume calculation for pelagic and benthic microalgae. J Phycol 35:403–424CrossRefGoogle Scholar
  24. Hoffmann LJ et al (2006) Different reactions of Southern Ocean phytoplankton size classes to iron fertilization. Limnol Oceanogr 51:1217–1229Google Scholar
  25. Hoffmann LJ et al (2007) Effects of iron on the elemental stoichiometry during EIFEX and in the diatoms Fragilariopsis kerguelensis and Chaetoceros dichaeta. Biogeosciences 4:569–579Google Scholar
  26. Hutchins DA et al (2001) Control of phytoplankton growth by iron and silicic acid availability in the subAntarctic Southern Ocean: Experimental results from the SAZ project. J Geophys Res, 106:31559–31572CrossRefGoogle Scholar
  27. Jeffrey SW, Humphrey GF (1975) New spectrophotometric equations for determining chlorophylls a, b, c1 and c2 in higher plants, algae and natural phytoplankton. Biochem Physiol Pflanzen 167:191–194Google Scholar
  28. Kolbowski J, Schreiber U (1995) Computer-controlled phytoplankton analyzer based on 4-wavelengths PAM chlorophyll fluorometer. In: Mathis P (ed) Photosynthesis: from light to biosphere, pp 825–828Google Scholar
  29. Leblanc K et al (2005) Fe and Zn effects on the Si cycle and diatom community structure in two contrasting high and low-silicate HNLC areas. Deep Sea Res Part I 52:1842–1864CrossRefGoogle Scholar
  30. Leynaert A et al (2004) Effect of iron deficiency on diatom cell size and silicic acid uptake kinetics. Limnol Oceanogr 49:1134–1143Google Scholar
  31. Martin-Jézéquel V et al (2000) Silicon metabolism in diatoms: implications for growth. J Phycol 36:821–840CrossRefGoogle Scholar
  32. Martin JH et al (1990) Iron deficiency limits phytoplankton growth in Antarctic waters. Global Biogeochem Cycles 4:5–12CrossRefGoogle Scholar
  33. Mitchell BG et al (1991) Light limitation of phytoplankton biomass and macronutrient utilization in the Southern Ocean. Limnol Oceanogr 36:1662–1677Google Scholar
  34. Montagnes DJS et al (1994) Estimating carbon, nitrogen, protein, and chlorophyll a from volume in marine phytoplankton. Limnol Oceanogr 39:1044–1060Google Scholar
  35. Morel FMM et al (1991) Limitation of productivity by trace metals in the sea. Limnol Oceanogr 36:1742–1755Google Scholar
  36. Nelson DM, Smith WO Jr (1991) Sverdrup revisited: critical depth, maximum chlorophyll levels, and the control of Southern Ocean productivity by the irradiance-mixing regime. Limnol Oceanogr 36:1650–1661Google Scholar
  37. Nelson DM et al (1995) Production and dissolution of biogenic silica in the ocean: revised global estimates, comparison with regional data and relationship to biogenic sedimentation. Global biogeochem Cycles 9:359–372CrossRefGoogle Scholar
  38. Paasche E, Østergren I (1980) The annual cycle of plankton diatom growth and silica production in the inner Oslofjord. Limnol Oceanogr 25:481–494CrossRefGoogle Scholar
  39. Raven JA (1990) Predictions of Mn and Fe use efficiencies of phototrophic growth as a function of light availability for growth and of C assimilation pathway. New Phytol 116:1–18CrossRefGoogle Scholar
  40. Sarthou G et al (2005) Growth physiology and fate of diatoms in the ocean: a review. J Sea Res 53:25–42CrossRefGoogle Scholar
  41. Sedwick PN et al (2002) Resource limitation of phytoplankton growth in the Crozet Basin, subAntarctic Southern Ocean. Deep Sea Res Part II 49:3327–3349CrossRefGoogle Scholar
  42. Sigmon DE et al (2002) The Si cycle in the Pacific sector of the Southern Ocean: seasonal diatom production in the surface layer and export to the deep sea. Deep Sea Res Part II 49:1747–1763CrossRefGoogle Scholar
  43. Strzepek RF, Price NM (2000) Influence of irradiance and temperature on the iron content of the marine diatom Thalassiosira weissflogii (Bacillariophyceae). Mar Ecol Prog Ser 206:107–117CrossRefGoogle Scholar
  44. Strzepek RF, Harrison PJ (2004), Photosynthetic architecture differs in coastal and oceanic diatoms. Nature 431:689–692PubMedCrossRefGoogle Scholar
  45. Sunda WG, Huntsman SA (1997) Interrelated influence of iron, light and cell size on marine phytoplankton growth. Nature 390:389–392CrossRefGoogle Scholar
  46. Thomas DN, Dieckmann GS (2002) Antarctic Sea Ice—a habitat for extremophiles. Science 295:641–644PubMedCrossRefGoogle Scholar
  47. Timmermans KR et al (2001) Co-limitation by iron and light of Chaetoceros brevis, C. dichaeta and C. calcitrans (Bacillariophyceae). Mar Ecol Prog Ser 217:287–297CrossRefGoogle Scholar
  48. Tréguer P, Jacques G (1992) Dynamics of nutrients and phytoplankton, and fluxes of carbon, nitrogen, and silicon in the Antarctic Ocean. Polar Biol 12:149–162CrossRefGoogle Scholar
  49. Tréguer P et al (1995) The silica balance in the world ocean: a reestimate. Science 268:375–379PubMedCrossRefGoogle Scholar
  50. Tsuda A et al (2003) A mesoscale iron enrichment in the western subArctic Pacific induces a large centric diatom bloom. Science 300:958–961PubMedCrossRefGoogle Scholar
  51. van Oijen T et al (2004) Light rather than iron controls photosynthate production and allocation in Southern Ocean phytoplankton populations during austral autumn. J Plankton Res 26:885–900CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.Leibniz Institute of Marine Science at the University of KielKielGermany
  2. 2.Department of Plant and Environmental SciencesGöteborg UniversityGöteborgSweden
  3. 3.Alfred-Wegener Institute for Polar and Marine ResearchBremerhavenGermany

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