Polar Biology

, 30:31 | Cite as

Response of Cyanobacteria and Algae from Antarctic Wetland Habitats to Freezing and Desiccation Stress

Original Paper


Antarctic wetlands are characterized by the presence of liquid water during short austral summer. Filamentous cyanobacteria are often dominant there and are exposed to severe conditions, of which the changes in the desiccation–rehydration and freeze–thaw cycles are two of the most stressful. Vigor, after freezing and desiccation, was laboratory tested in cyanobacterial and algal strains from wetland habitats collected in maritime and continental Antarctica. Whereas minor sub-zero temperatures (−4°C), demonstrating summer diurnal freeze–thaws did not cause significant damage on either cyanobacteria or algae, low sub-zero temperatures (−40, −100, −196°C), demonstrating annual winter freeze, caused little harm to cyanobacteria, but was fatal for more than 50% of the population of algae. Freezing and desiccation tolerance of these strains was compared using multiregression methods: cyanobacteria from continental Antarctica were significantly more tolerant to low sub-zero temperatures than similar strains from maritime Antarctica (P = 0.026; F = 3.66); and cyanobacteria from seepages habitat were less tolerant to freezing and desiccation than cyanobacteria from other wetlands (P = 0.002; F = 5.69).



This work was supported by the EU project QLRT-2000-01645 (COBRA), the GA CR project (206/05/0253) and grant MSM 143100007. We are also very grateful to captain Stuart Lawrence and his team of the British Antarctic Survey Research Vessel “Ernest Shackleton” for their warm hospitality and friendship during Josef Elster’s stay on board.


  1. Becker EW (1982) Physiological studies on Antarctic Prasiola crispa and Nostoc commune at low temperatures. Polar Biol 1:99–104Google Scholar
  2. ter Braak CJF (1990) CANOCO: a fortran program for canonical community ordination: update notes. Agricultural Mathematics Group, WageningenGoogle Scholar
  3. ter Braak CJF, Šmilauer P (2002) CANOCO reference manual and CanoDraw for Windows user’s guide: software for canonical community ordination (version 4.5). Microcomputer Power, IthacaGoogle Scholar
  4. Davey MC (1989) Te effect of freezing and desiccation on photosynthesis and survival of terrestrial Antarctic algae and cyanobacteria. Polar Biol 10:29–36CrossRefGoogle Scholar
  5. Davey MC, Pickup J, Block W (1992) Temperature variation and its biological significance in fellfield habitats on a maritime Antarctic island. Antarct Sci 4:383–388Google Scholar
  6. Elster J (2002) Ecological classification of terrestrial algal communities in polar environments. In: Beyer L, Bölter M (eds) Geoecology of Antarctic ice-free coastal landscapes. Springer, Berlin Heidelberg New York, pp 303–326Google Scholar
  7. Elster J, Svoboda J (1996) Algal seasonality and abundance in, and along, glacial stream Sverdrup Pass 79°N, Central Ellesmere Island, Canada. Mem Natl Inst Polar Res Spec Issue 51:99–118Google Scholar
  8. Elster J, Benson EE (2004) Life in the polar terrestrial environment with a focus on algae and cyanobacteria. In: Fuller B, Lane N, Benson E (eds) Life in the frozen state. Taylor and Francis London, pp 110–150Google Scholar
  9. Elster J, Svoboda J, Komárek J, Marvan P (1997) Algal and cyanoprocaryote communities in a glacial stream, Swerdrup Pass 79°N, central Ellesmere Island, Canada. Arch Hydrobiol Suppl 85:57–93Google Scholar
  10. Elster J, Lukešová A, Svoboda J, Kopecký J, Kanda H (1999) Diversity and abundance of soil algae in the polar desert, Sverdrup Pass, central Ellesmere Island. Polar Rec 35:231–254CrossRefGoogle Scholar
  11. Hawes I (1990) Effect of freezing and thawing on a species of Zygnema (Chlorophyta) from the Antarctic. Phycologia 29:326–331Google Scholar
  12. Hawes I, Howard-Williams C, Vincent WF (1992) Desiccation and recovery of Antarctic cyanobacterial mats. Polar Biol 12:587–594CrossRefGoogle Scholar
  13. Jacob A, Wiencke C, Lehmann H, Krist GO (1992) Physiology and ultrastructure of desiccation in the green alga Prasiola crispa from Antarctica. Bot Mar 35:297–303Google Scholar
  14. Komárek O, Komárek J (1999) Diversity of freshwater and terrestrial habitats and their oxyphototroph microflora in the Arctowski Station region, South Shetlands Islands. Pol Polar Res 20:259–282Google Scholar
  15. Komárek J, Komárek O (2003) Diversity of cyanobacteria in seepages of King George Island, maritime Antarctica. In: Huiskes AHL, Gieskes WWC, Rozema J, Schorno RML, van der Vies SM, Wolff WJ (eds) Antarctic biology in a global context, Proceedings of the VIII SCAR international biology symposium. Backhuys, Leiden, pp 244–250Google Scholar
  16. Lukavský J (1975) Analysis of growth rate of algae by cultivation on solid media. Arch Hydrobiol Suppl 14:105-136Google Scholar
  17. Stanier RY, Kunisawa R, Mandel M, Cohen-Bazire G (1971) Purifcation and properties of unicellular blue-green algae (order Chroococcales). Bacteriol Rev 35:171–205PubMedGoogle Scholar
  18. Stibal M, Elster J (2005) Growth and morphology variation as a response to changing environmental factors in two Arctic species of Raphidonema (Trebouxiophyceae) from soil and snow. Polar Biol 28:558–567CrossRefGoogle Scholar
  19. Tang EPY, Tremblay R, Vincent WF (1997) Cyanobacterial dominance of polar freshwater ecosystems: are high-latitude mat-formers adapted to low temperature? J Phycol 33:171–181CrossRefGoogle Scholar
  20. Taylor R, Fletcher RL (1999) Cryopreservation of eukaryotic algae—a review of methodologies. J Appl Phycol 10:481–501CrossRefGoogle Scholar
  21. Vincent WF, Downes MT, Castenholz RW, Howard-Williams C (1993) Community structure and pigment organisation of cyanobacteria-dominated microbial mats in Antarctica. Eur J Phycol 28:213–221Google Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  1. 1.Institute of BotanyAcademy of Sciences of the Czech RepublicTrebonCzech Republic
  2. 2.Faculty of Biological SciencesUniversity of South BohemiaCeske BudejoviceCzech Republic

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