Plant Cell Reports

, Volume 19, Issue 8, pp 798–803

The use of phosphomannose-isomerase as a selectable marker to recover transgenic maize plants (Zea mays L.) via Agrobacterium transformation

  • D. Negrotto
  • M. Jolley
  • S. Beer
  • A. R. Wenck
  • G. Hansen
GENETIC TRANSFORMATION AND HYBRIDIZATION

DOI: 10.1007/s002999900187

Cite this article as:
Negrotto, D., Jolley, M., Beer, S. et al. Plant Cell Reports (2000) 19: 798. doi:10.1007/s002999900187

Abstract

A new selectable marker system has been adapted for use in Agrobacterium-mediated transformation of maize. This selection system utilizes the pmi gene encoding for phosphomannose-isomerase that converts mannose-6-phosphate to fructose-6-phosphate. Only transformed cells are capable of utilizing mannose as a carbon source. Agrobacterium-mediated transformation of immature embryos followed by a pre-selection of 10–14 days prior to selection at a level of 1% mannose and 0.5% sucrose led to the recovery of trangenic lines of a frequency of as high as 30% in about 12 weeks. Molecular and genetic analysis showed that selected plants contained the pmi gene and that the gene was transmitted to the progeny in a Mendelian fashion.

Key words Maize transformation Phosphomannose-isomerase Positive selection 

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • D. Negrotto
    • 1
  • M. Jolley
    • 1
  • S. Beer
    • 1
  • A. R. Wenck
    • 1
  • G. Hansen
    • 1
  1. 1.Novartis Agribusiness Biotechnology Research Inc, NABRI, 3054 Cornwallis Road, Research Triangle Park, NC 27709-2257, USA e-mail: genevieve.hansen@nabri.novartis.com Fax: +919-541-8585WF

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