Plant Cell Reports

, Volume 20, Issue 5, pp 376–382 | Cite as

The utility of green fluorescent protein in transgenic plants

  • C. Stewart
Review

Abstract.

The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has proven to be a powerful tool in plant genetic transformation studies. This paper reviews the history and the progression of the expression of GFP variants in transgenic plants. The distinguishing features of the most useful GFPs, such as those including the S65T chromophore mutation and those with dual excitation peaks, are discussed. The review also focuses on the utility of GFP as a visual selectable marker in aiding the plant transformation process; GFP has been more important in monocot transformation compared with dicot transformation. Finally, the potential utility of new fluorescent proteins is speculated upon.

Fluorescent proteins GFP Gene expression Marker genes Plant transformation 

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • C. Stewart
    • 1
  1. 1.Department of Biology, University of North Carolina-Greensboro, NC 27402-6174, USAUSA

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