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Plant Cell Reports

, Volume 19, Issue 5, pp 529–534 | Cite as

Development of a plant regeneration system based on friable embryogenic callus in the ornamental Alstroemeria

  • H. -S. Lin
  • C. van der Toorn
  • K. J. J. M. Raemakers
  • R. G. F. Visser
  • M.J. De Jeu
  • E. Jacobsen
Original paper

Abstract

 Stem segments of seedlings from two Alstroemeria breeding lines, cultured on media supplemented with 4 mg/l 2,4-dichlorophenoxyacetic acid and 0.5–1.0 mg/l 6-benzylaminopurine (BA), initiated soft callus, which became compact after subculture on a medium with only 0.5 mg/l BA. Friable embryogenic calli were initiated from compact callus on a medium supplemented with 10 mg/l picloram. Proembryos developed from friable embryogenic calli via embryos into plants after subculture on medium supplemented with 0.1 mg/l BA. The proembryos formed friable embryogenic calli again after culture on medium supplemented with 10 mg/l picloram. The total time needed to regenerate a complete plantlet from friable callus was approximately 6 months. This system for the production of embryogenic material is considered to have valuable applications for genetic transformation in Alstroemeria.

Key words Friable embryogenic callus Regeneration Rhizome Alstroemeria 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • H. -S. Lin
    • 1
  • C. van der Toorn
    • 1
  • K. J. J. M. Raemakers
    • 1
  • R. G. F. Visser
    • 1
  • M.J. De Jeu
    • 1
  • E. Jacobsen
    • 1
  1. 1.Graduate School of Experimental Plant Sciences, Laboratorium of Plant Breeding, Wageningen Agricultural University, P.O. Box 386, 6700 AJ Wageningen, The NetherlandsNL

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