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Plant Cell Reports

, Volume 19, Issue 5, pp 459–463 | Cite as

Efficient plant regeneration of Mesembryanthemum crystallinum via somatic embryogenesis

  • J. C. Cushman
  • T. Wulan
  • N. Kuscuoglu
  • M. D. Spatz
Original paper

Abstract

 An efficient plant regeneration procedure has been established from hypocotyl explants of the common ice plant, Mesembryanthemum crystallinum L, a halophytic leaf succulent that exhibits a stress-induced switch from C3 photosynthesis to crassulacean acid metabolism (CAM). Somatic embryos were initiated and developed up to globular and heart stages in Murashige and Skoog (MS) media supplemented with 3% sucrose, 0.6% bacto-agar, 80 mM NaCl, 5 μM 2,4-D and 1 μM kinetin. High frequency regeneration occurred when somatic embryos were germinated on media that lacked 2,4-D. High cytokinin treatment suppressed normal growth of embryos and favored abnormal embryo proliferation. Without growth regulators, regenerated plants rooted on MS medium with 100% efficiency. Mature, regenerated plants were fertile and morphologically identical to seed-derived plants.

Key words Mesembryanthemum crystallinum Aizoaceae Somatic embryogenesis Halophyte Facultative CAM plant 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • J. C. Cushman
    • 1
  • T. Wulan
    • 1
  • N. Kuscuoglu
    • 2
  • M. D. Spatz
    • 1
  1. 1.Department of Biochemistry and Molecular Biology, 147 Noble Research Center, Oklahoma State University, Stillwater, Oklahoma, USA Fax: +1–405–744–7799 e-mail: jcushman@biochem.okstate.eduUS
  2. 2.Department of Biochemistry, 540 Biosciences West, University of Arizona, Tucson, AZ 85721 USAUS

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