Plant Cell Reports

, Volume 19, Issue 2, pp 111–117 | Cite as

Direct sowing of Coffea arabica somatic embryos mass-produced in a bioreactor and regeneration of plants

  • D. Etienne-Barry
  • B. Bertrand
  • N. Vasquez
  • H. Etienne
Original paper

Abstract

 The effect of germination conditions on the morphology of Coffea arabica L. somatic embryos mass-produced in a 1-l temporary immersion bioreactor (RITA®) was studied with emphasis on direct sowing in soil. Using germinated embryos, direct sowing resulted in a highly successful conversion of embryos into plants. A culture density above 1600 embryos per 1-l bioreactor positively affected embryo morphology by causing higher embryonic axis elongation (+4–5 mm). At this density, the addition of a high concentration of sucrose (234 mM) 2 weeks before sowing promoted an increase in effective plant conversion in soil (78%) and a vigorous vegetative growth of the resulting plants. Furthermore, direct sowing reduced handling time to 13% and shelving area requirements to 6.3% of the values obtained by conventional acclimatization of plants developed on gel media.

Key words Acclimatization Bioreactor Coffee Somatic embryogenesis Plant regeneration 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • D. Etienne-Barry
    • 2
  • B. Bertrand
    • 3
  • N. Vasquez
    • 2
  • H. Etienne
    • 1
  1. 1.CIRAD-CP/PROMECAFE/CATIE, Apartado 11, CATIE 7170 Turrialba, Costa Rica e-mail: hetienne@catie.ac.cr Fax: +506-556-09-38CR
  2. 2.Laboratorio de biotecnología, CATIE, 7170 Turrialba, Costa RicaCR
  3. 3.CIRAD-CP/PROMECAFE/IICA, Ap. 55, 2200 San José, Costa RicaCR

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