Plant Cell Reports

, Volume 18, Issue 10, pp 835–840

Resistance to Botrytis cinerea in scented geranium transformed with a gene encoding the antimicrobial protein Ace-AMP1

  • Y.-M. Bi
  • B. P. A. Cammue
  • P. H. Goodwin
  • S. KrishnaRaj
  • P. K. Saxena

DOI: 10.1007/s002990050670

Cite this article as:
Bi, YM., Cammue, B., Goodwin, P. et al. Plant Cell Reports (1999) 18: 835. doi:10.1007/s002990050670

Abstract

Scented geranium (Pelargonium sp. `Fren-sham') was transformed with a gene encoding an antimicrobial protein (Ace-AMP1) from onion through an Agrobacterium-mediated transformation system. The binary vector pFAJ3033 contained the coding region of the Ace-AMP1 preproprotein-encoding cDNA. Transformants were verified by polymerase chain reaction and Southern blot analysis. Transgenic plants expressing high levels of Ace-AMP1 were identified by immunoblots and those plants were shown to have increased resistance to Botrytis cinerea leaf infection.

Key words Geranium Genetic transformation Ace-AMP1 Botrytis cinerea Disease resistance 

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Y.-M. Bi
    • 1
  • B. P. A. Cammue
    • 2
  • P. H. Goodwin
    • 3
  • S. KrishnaRaj
    • 1
  • P. K. Saxena
    • 1
  1. 1.Department of Horticultural Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1 Fax: 519-767-0755 e-mail: psaxena@uoguelph.caCA
  2. 2.F. A. Janssens Laboratory of Genetics, Katholieke Universiteit Leuven, K. Mercierlaan 92, B-3001 Heverlee, BelgiumBE
  3. 3.Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1CA

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