Plant Cell Reports

, Volume 18, Issue 9, pp 707–714

Green-fluorescent protein facilitates rapid in vivo detection of genetically transformed plant cells

  • A. R. Elliott
  • J. A. Campbell
  • B. Dugdale
  • R. I. S. Brettell
  • C. P. L. Grof

Abstract

Early detection of plant transformation events is necessary for the rapid establishment and optimization of plant transformation protocols. We have assessed modified versions of the green fluorescent protein (GFP) from Aequorea victoria as early reporters of plant transformation using a dissecting fluorescence microscope with appropriate filters. Gfp-expressing cells from four different plant species (sugarcane, maize, lettuce, and tobacco) were readily distinguished, following either Agrobacterium-mediated or particle bombardment-mediated transformation. The identification of gfp-expressing sugarcane cells allowed for the elimination of a high proportion of non-expressing explants and also enabled visual selection of dividing transgenic cells, an early step in the generation of transgenic organisms. The recovery of transgenic cell clusters was streamlined by the ability to visualize gfp-expressing tissues in vitro.

Key words Green-fluorescent protein Transformation Particle bombardment Agrobacterium Sugarcane 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • A. R. Elliott
    • 1
  • J. A. Campbell
    • 1
  • B. Dugdale
    • 2
  • R. I. S. Brettell
    • 3
  • C. P. L. Grof
    • 1
  1. 1.CSIRO Tropical Agriculture, 120 Meiers Rd, Indooroopilly, Queensland 4068, Australia Fax: +61-7-32142288 e-mail: chris.grof@tag.csiro.auAU
  2. 2.School of Life Sciences, Queensland University of Technology, GPO Box 2434, Brisbane, Queensland 4001, AustraliaAU
  3. 3.CSIRO Plant Industry, Darwin Laboratory, PMB 44, Winnellie, NT 0822, AustraliaAU

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