Advertisement

Plant Cell Reports

, Volume 18, Issue 6, pp 456–461 | Cite as

In vitro multiplication of cashew (Anacardium occidentale L.) using shoot node explants of glasshouse-raised plants

  • B. Boggetti
  • J. Jasik
  • S. Mantell

Abstract

Using glasshouse-raised plants (1 month, 1 year and 5 years old), factors affecting shoot development from shoot nodes of two Brazilian and one Tanzanian elite selections of cashew (Anacardium occidentale L.) were assessed. Sprouting of buds decreased strongly with increasing age of mother plants. Solidified media, mainly when purified agar was used, gave better results than liquid medium. Murashige and Skoog salts containing 1/2-strength macroelements were the most suitable for bud sprouting and shoot elongation. Vitamins and sucrose concentration did not have a significant effect but by replacing 20 g/l sucrose with glucose or maltose all estimated parameters were improved. Gibberellins supported bud sprouting and shoot elongation but blocked rooting. Shoots developed in the presence of cytokinins were short and produced axillary branches. Activated charcoal, cultivation of explants in darkness for the first 7 days and superoptimal temperature (35 °C) decreased bud sprouting and supported shoot elongation. Microshoots rooted in vitro at a frequency of 42% when cultured for 5 days with 100 μμ indole-3-butyric acid. Over 40% of rooted microshoots survived weaning.

Key wordsAnacardium occidentale Bud sprouting Cashew Shoot nodes Shoot elongation 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • B. Boggetti
    • 1
  • J. Jasik
    • 2
  • S. Mantell
    • 1
  1. 1.Unit for Advanced Propagation Systems, Horticulture Section, Wye College, University of London, Wye, Ashford, TN25 5AH, UKGB
  2. 2.Department of Plant Physiology, Comenius University, Mlynska dolina B2, 84215 Bratislava, SlovakiaXX

Personalised recommendations