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Plant Cell Reports

, Volume 18, Issue 3–4, pp 341–345 | Cite as

The biosynthetic relationship between littorine and hyoscyamine in transformed roots of Datura stramonium

  • I. Zabetakis
  • R. Edwards
  • John T. G. Hamilton
  • D. O'Hagan

Abstract

The metabolic relationship between littorine and hyoscyamine has been monitored in transformed roots of Datura stramonium. Quantification by GC of unlabelled littorine and by GCMS of 13C-labelled littorine demonstrated that exogenously added littorine (0.1 mm) was significantly metabolised (35%) to hyoscyamine. In contrast, exogenously added hyoscyamine was not metabolised to littorine, indicating that this conversion is irreversible. The conversion of littorine to hyoscyamine was suppressed by P-450 oxidase inhibitors (particularly clotrimazole), implicating the involvement, at least in part of a cytochrome P-450 activity operating hyoscyamine biosynthesis.

Key wordsDatura stramonium Tropane alkaloids Hyoscyamine Biosynthesis 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • I. Zabetakis
    • 1
  • R. Edwards
    • 2
  • John T. G. Hamilton
    • 3
  • D. O'Hagan
    • 1
  1. 1.Department of Chemistry, Science Laboratories, University of Durham, South Road, Durham, DH1 3LE, UKGB
  2. 2.Department of Biological Sciences, University of Durham, Durham, DH1 3LE, UKGB
  3. 3.Department of Food Science, The Queen's University of Belfast, Newforge Lane, Belfast, BT9 5PX, UKGB

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