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Plant Cell Reports

, Volume 18, Issue 1–2, pp 25–31 | Cite as

A protocol for consistent, large-scale production of fertile transgenic rice plants

  • L. Chen
  • S. Zhang
  • R. N. Beachy
  • C. M. Fauquet
Article

Abstract

A protocol for consistent production of fertile transgenic rice plants was established utilizing microparticle bombardment of embryogenic tissues (Oryza sativa L. japonica cv. Taipei 309). This system has been employed to produce several thousand independently transformed plant lines carrying the hygromycin phosphotransferase (hph) gene and various genes of interest. The most efficient target tissue was highly embryogenic callus or suspension cell aggregates, when they were given an osmotic pre- and post-transformation treatment of 0.6 m carbohydrate. By optimizing the age of the tissue at the time of gene transfer and applying an improved selection procedure, transgenic plants were recovered in 8 weeks from the time of gene transfer, at an average of 22.3±9.7 per 100 calli and 22.4±8.0 plant lines per dish of suspension cell aggregates. This system has facilitated a number of studies using rice as a model for genetic transformation and will enable the large-scale production of transgenic rice plants for genomic studies.

Key words

Rice Microparticle bombardment Genetic transformation Embryogenic tissues 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • L. Chen
    • 1
  • S. Zhang
    • 1
  • R. N. Beachy
    • 1
  • C. M. Fauquet
    • 1
  1. 1.International Laboratory for Tropical Agricultural Biotechnology (ILTAB/ORSTOM-TSRI), Division of Plant Biology-BCC 206The Scripps Research InstituteLa JollaUSA

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