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Plant Cell Reports

, Volume 17, Issue 6–7, pp 489–496 | Cite as

Co-transformation with one Agrobacterium tumefaciens strain containing two binary plasmids as a method for producing marker-free transgenic plants

  • M. Daley
  • V. C. Knauf
  • K. R. Summerfelt
  • J. C. Turner

Abstract

Co-transformation was investigated as a method that would allow the use of a selectable marker during plant regeneration followed by recovery of progeny which contain the desired gene(s) but lack a marker gene. Rapeseed (Brassica napus cv `212/86') and tobacco (Nicotiana tabacum cv `Xanthi NC') were co-cultivated with a single Agrobacterium tumefaciens strain containing two binary plasmids. Genes from both plasmids were expressed in approximately 50% of the primary transformants. Progeny expressing only one of the transgenes were observed in about 50% of the co-transformed lines, indicating that the genes were inserted at different loci. This single-strain co-transformation method allowed the use of a selectable marker during plant regeneration and subsequent recovery of marker-free progeny.

Key words Co-transformation Marker-free plants Brassica Rapeseed Tobacco 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • M. Daley
    • 1
  • V. C. Knauf
    • 1
  • K. R. Summerfelt
    • 1
  • J. C. Turner
    • 1
  1. 1.Calgene Inc., 1920 Fifth St., Davis, CA 95616, USAUS

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