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Plant Cell Reports

, Volume 17, Issue 6–7, pp 446–450 | Cite as

Callus induction and somatic embryogenesis of Phalaenopsis

  • Y. Ishii
  • T. Takamura
  • M. Goi
  • M. Tanaka

Abstract

Callus induction and plant regeneration through somatic embryogenesis in Phalaenopsis Richard Shaffer `Santa Cruz' were examined. Protocorm-like body (PLB) segments formed calli in Vacin and Went medium with sucrose. The optimal concentration of sucrose was 40 g ⋅ l–1. Medium containing 200 ml ⋅ l–1 coconut water together with 40 g ⋅ l–1 sucrose was effective for callus induction. Gellan gum was suitable than agar as a gelling agent for callus induction. The calli easily formed PLBs after being transferred to a medium without sucrose. Histological observation suggested that the PLBs were somatic embryos. No variation was observed in the flowering plants regenerated through somatic embryogenesis.

Key wordsPhalaenopsis Protocorm-like bodies Callus induction Somatic embryogenesis 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Y. Ishii
    • 1
  • T. Takamura
    • 1
  • M. Goi
    • 1
  • M. Tanaka
    • 1
  1. 1.Department of Horticulture, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795, JapanJP

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