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Plant Cell Reports

, Volume 17, Issue 4, pp 251–255 | Cite as

Plant regeneration from callus culture of Cymbidium ensifolium var. misericors

  • C. Chang
  • W. C.  Chang

Abstract

Totipotent calli of Cymbidium ensifolium var. misericors, a locally grown orchid of high commercial value, were induced from sections of pseudobulbs, rhizomes and roots of seed-derived plantlets on 1/2-strength Murashige and Skoog medium plus 10 mg/l 2,4-dichlorophenoxyacetic acid and 0.1 mg/l thiadiazuron. The calli could be maintained by subculturing in the same medium. The calli could be induced to develop further along one of three distinct morphogeneic routes: (1) production of rhizomes, (2) production of shoot buds, or (3) development of granular embryoids. Efficient mass propagation was possible via rhizome proliferation and embryoid formation.

Key wordsCymbidium ensifolium var. misericors Plant regeneration Rhizome Somatic embryogenesis Totipotent callus 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • C. Chang
    • 1
  • W. C.  Chang
    • 1
  1. 1.Institute of Botany, Academia Sinica, Taipei, Taiwan 115, Republic of China Fax no.: +886-2-7827954 E-mail: wcchang@wcc.sinica.edu.twTW

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