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Plant Cell Reports

, Volume 17, Issue 3, pp 172–176 | Cite as

Plant regeneration from encapsulated somatic embryos of Carica papaya L.

  • B. Castillo
  • M. A. L. Smith
  • U. L. Yadava

Abstract

Carica papaya L. (papaya) single somatic embryos (2.0 mm diameter) produced in a high-frequency liquid production system were encapsulated in two different synthetic encapsulation compounds. The frequency of regeneration from encapsulated embryos was significantly affected by (1) the concentration of sodium alginate, (2) the presence or absence of nutrient salts in the capsule, and (3) the duration of exposure to calcium chloride. A 2.5% sodium alginate concentration in a half-strength MS salts base resulted in significantly higher germination frequencies than other treatments. A relatively short (10 min) exposure to CaCl2 provided uniform encapsulation of embryos and the highest frequencies of successful germination (77.5%). Germinated artificial seeds produced normal plantlets.

Key words Papaya Somatic embryogenesis Synthetic seed 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • B. Castillo
    • 1
  • M. A. L. Smith
    • 1
  • U. L. Yadava
    • 2
  1. 1.Department of Natural Resources and Environmental Sciences, 1021 Plant Sciences Laboratory, 1201 S Dorner Dr., MC-634, University of Illinois, Urbana, IL 61801-4778, USA Fax-no.: +1-217-244-3469 E-mail: imagemal@uiuc.eduUS
  2. 2.Department of Plant Sciences, Georgia Agricultural Experiment Stations, Fort Valley State University, Fort Valley, GA 31030-3298, USAGE

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