Advertisement

Plant Cell Reports

, Volume 17, Issue 1, pp 68–72 | Cite as

High-frequency in vitro multiplication of disease-free Zingiber officinale Rosc.

  • T. R. Sharma
  • B. M. Singh

Abstract

High-frequency in vitro multiplication of disease-free clones of ginger (Zingiber officinale Rosc.) was obtained by culturing small and active buds of ginger on MS medium supplemented with 2 mg/l Kin and 20 g/l sucrose. An average of 7.7 shoots per bud was obtained on this medium after 4 weeks of culture. A high multiplication rate of well-developed plantlets (7.0 shoots per bud) with a 6.8-cm shoot length and a 7.0-cm root length was also obtained on MS medium containing 2.0 mg Kin, 2.0 mg NAA and 20 g sucrose per liter. The multiplication rate did not decrease even up to 28 months of subculture on the same medium. A simple method of successfully transferring more than 95% of tissue-cultured plants into pots was also standardized. In vitro-derived plants performed well under field conditions, were morphologically identical to the mother plants and were free from ginger yellows (Fusarium oxysporum f. sp. zingiberi). Well-developed rhizomes obtained from the tissue-cultured plants did not rot during storage of up to 6 months, thus indicating that the method is also effective in checking storage rot caused by F. oxysporum f. sp. zingiberi.

Key wordsFusarium oxysporum f. sp. zingiberi Ginger Tissue culture Micropropagation 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • T. R. Sharma
    • 1
  • B. M. Singh
    • 1
  1. 1.Biotechnology Centre, Himachal Pradesh Krishi Vishvavidyalaya, Palampur-176062, IndiaIN

Personalised recommendations