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Plant Cell Reports

, Volume 16, Issue 11, pp 770–774 | Cite as

Direct shoot regeneration from excised leaf explants of in vitro grown seedlings of Alstroemeria L.

  • H. S. Lin
  • M. J.  De Jeu
  • E. Jacobsen

Abstract

A two-step protocol for the induction of shoots from Alstroemeria leaf explants has been developed. Leaf explants with stem node tissue attached were incubated on shoot induction medium for 10 days, and then transferred to regeneration medium. Shoots from the area adjacent to the region between the leaf base and node tissue regenerated within 3 weeks after transfer to the regeneration medium, without a callus phase. The best induction was obtained with Murashige and Skoog medium containing 10 µm thidiazuron and 0.5 µm indole butyric acid. The regeneration medium contained 2.2 µm 6-benzylaminopurine. After several subcultures of the leaf explants with induced shoots, normal plantlets with rhizome were formed. In Alstroemeria, the percentage of responding leaf explants is more important than the number of shoots regenerated per leaf explant, because rhizome formation is the most important factor for micropropagation. The effect of other compounds in the induction medium, including glucose, sucrose, silver nitrate, and ancymidol, on regeneration was also investigated.

Key wordsAlstroemeria Regeneration Thidiazuron Silver nitrate Ancymidol 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • H. S. Lin
    • 1
  • M. J.  De Jeu
    • 1
  • E. Jacobsen
    • 1
  1. 1.The Graduate School Experimental Plant Sciences, Department of Plant Breeding, Wageningen Agricultural University (WAU), PO Box 386, 6700 AJ Wageningen, The NetherlandsNL

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