Plant Cell Reports

, Volume 16, Issue 10, pp 668–673 | Cite as

Transfer of a grapevine stilbene synthase gene to rice (Oryza sativa L.)

  • P. Stark-Lorenzen
  • B. Nelke
  • G. Hänßler
  • H. P. Mühlbach
  • J. E. Thomzik

Abstract

A gene derived from grapevine (Vitis vinifera) coding for stilbene synthase has been transferred into protoplasts of the commercially important japonica rice cultivar Nipponbare using PEG-mediated direct gene transfer. Transgenic plants were regenerated from calli selected on kanamycin. Southern blot analysis of genomic DNA isolated from regenerants and progeny plants demonstrated that the stilbene synthase gene is stably integrated in the genome of transgenic rice plants and inherited in the offspring. The transient formation of stilbene-synthase-specific mRNA shortly after inoculation with the fungus of the rice blast Pyricularia oryzae has demonstrated that the grapevine stilbene synthase promoter is also active in monocotyledonous plants. Preliminary results indicate an enhanced resistance of transgenic rice to P. oryzae.

Key wordsOryza sativa Pyricularia oryzae Transformation Stilbene synthase Phytoalexin 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • P. Stark-Lorenzen
    • 1
  • B. Nelke
    • 2
  • G. Hänßler
    • 2
  • H. P. Mühlbach
    • 3
  • J. E. Thomzik
    • 2
  1. 1.Max-Planck-Institut für Züchtungsforschung, D-50829 Köln, GermanyDE
  2. 2.Agrochemical Division, Research/Biotechnology, Bayer AG, D-51368 Leverkusen-Bayerwerk, GermanyDE
  3. 3.Institut für Allgemeine Botanik, Universität Hamburg, Ohnhorststraße 18, D-22609 Hamburg, GermanyDE

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